化抑制剂酸盐和基质在草原土壤中对comammoxNitrospira产生不同的影响
Anish S Shah1, Pei-Chun Hsu1, Chris Chisholm1
1Centre for Soil and Environmental Research, Lincoln University, Lincoln, New Zealand.
Frontiers in microbiology
|May 29, 2024
概括
化抑制剂DCD和酸盐揭示了comammoxNitrospira,AOB和AOA在草原土壤中的不同作用. 酸盐对comammox Nitrospira产生了影响,而AOB对产生了反应,而AOA对产生了最小的反应.
科学领域:
- 土壤微生物学 土壤微生物学
- 生物地质化学循环是什么
- 氨的氧化方式是氨氧化.
背景情况:
- 亚化是一个关键的土壤过程,由氨氧化微生物驱动.
- 了解不同氨氧化剂 (comammoxNitrospira,AOB,AOA) 的作用对于土壤循环至关重要.
- 化抑制剂用于研究这些微生物群落和过程.
研究的目的:
- 研究comammoxNitrospira clade B,氨氧化细菌 (AOB) 和氨氧化古生物 (AOA) 在化中的作用.
- 评估二胺胺 (DCD) 和酸盐化抑制剂对这些微生物群体的影响.
- 为了检查这些氨氧化剂对草原土壤中不同水平的改剂的反应.
主要方法:
- 进行了一项为期90天的土壤化研究, (N) 含量变化 (0, 50, 700 mg-N kg-1).
- 土壤使用或不使用DCD和酸盐化抑制剂进行了处理.
- 使用定量PCR (qPCR) 来测量comammoxNitrospira,AOA,AOB和NOB基因的丰富性.
- 照明测序分析了comammox Nitrospira社区结构.
主要成果:
- 酸盐显著抑制了NH4+和NO2−的氧化,减少了comammox Nitrospira amoA和nxrB基因的丰度,并改变了社区结构.
- 随着N的添加,AOB种群增加,并被DCD和酸盐抑制.
- AOA对N或抑制剂的反应很小,而comammox Nitrospira clade B被高度的氨抑制.
结论:
- 康马莫克斯Nitrospira,AOB和AOA对的可用性和化抑制剂的反应不同.
- 酸盐在抑制comammoxNitrospira方面是有效的,而AOB对抑制剂和N添加都敏感.
- 需要进一步的研究来澄清DCD和酸盐在不同的氨氧化群体上的特定抑制机制.
相关概念视频
Overview of Nitrogen Metabolism
7.9K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.9K
The Nitrogen Cycle
52.0K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
52.0K
2° Amines to N-Nitrosamines: Reaction with NaNO2
4.2K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
4.2K
meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H
5.5K
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for...
5.5K
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
3.3K
Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
3.3K
The Roles of Bacteria and Fungi in Plant Nutrition
35.3K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
35.3K


