细菌amyloliquefaciens减弱肠道透性,氧化应激和内分泌网膜应激:断奶小猪的转录组和微生物组分析
Junmeng Yuan1, Hongling Meng1, Yu Liu1
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Frontiers in microbiology
|May 29, 2024
概括
乙氨基菌SC06补充剂通过减少氧化应激和内分泌网膜应激改善了小猪生长和肠道健康. 这种益生菌调节了肠道基因和微生物群,减轻了二引发的损伤.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 细胞内膜网膜 (ER) 的压力与氧化压力 (OS) 和肠道损伤有关.
- 众所周知,益生菌Bacillus amyloliquefaciens SC06 (SC06) 能够调节OS,但其对肠道ER压力的影响尚不清楚.
研究的目的:
- 调查SC06在减轻断奶小猪肠道ER压力和OS中的作用.
- 为了探索SC06对基因表达,肠道透性和叶微生物群组成在二引发的压力下的影响.
主要方法:
- 采用了一个2x2的因数设计实验,对32只断奶的小猪进行了实验.
- 治疗包括两种SC06水平 (0或1x10^8CFU/g) 和dquat (DQ) 注射 (或没有注射).
- 测量包括生长表现,阴茎透性,OS标志物,ER压力指标,阴茎转录组和微生物组分析.
主要成果:
- 补充SC06改善了DQ治疗小猪的生长性能,并降低了DQ治疗小猪的乳腺透性,OS和ER压力.
- 转录组分析显示,DQ诱导的基因在NF-κB信号通路中被丰富,而SC06+DQ治疗丰富了谷氨代谢通路.
- 叶微生物组分析显示,SC06+DQ治疗改变了微生物组成,减少了Clostridium和增加了Actinobacillus,发现了微生物群和ER压力基因之间的相关性.
结论:
- 食补充SC06可以提高断奶小猪的生长性能.
- SC06有效地降低了肠道的透性,氧化应激和内质网膜应激.
- SC06通过调节乳房内基因表达和调节肠道微生物群组成来发挥其保护作用.
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