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人类RNA作为精子质量和男性不孕不育的潜在生物标志物

Liping Cheng¹, He Jin¹, Tianheng Xiao¹

¹State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Journal of biomedical research

|May 29, 2024

概括

来自BOULE基因的丸特异性循环RNA (circRNAs) 显示为男性不孕症的生物标志物具有前途. 降低特定circBOULERNAs水平与精子质量差和DNA完整性相关,表明具有诊断潜力.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

预测男性生育能力的可靠分子生物标志物有限.
精子质量对于自然受孕和辅助生殖技术至关重要.
循环RNAs (circRNAs) 正在成为各种生物过程中的潜在调节分子.

研究的目的:

研究丸特定的circBOULERNAs作为男性不孕症的潜在生物标志物.
评估circBOULE RNA表达和精子质量参数之间的相关性.
探索circBOULERNAs在精子发育和生育中的功能作用.

主要方法:

用RT-PCR和RT-qPCR来识别和量化人类精子中的circBOULERNA.
在不育男性和对照者之间,比较了特定circRNAs的表达水平.
在circRNA水平和精子DNA碎片化指数 (DFI),受精率和裂变率之间进行了相关性分析.
在转基因模型中进行了功能研究.

主要成果:

在精子中发现了来自人类BOULE基因的7个circRNAs.
在精 (精子运动性低) 中观察到circEx3-6的表达减少.
在三精子症 (精子形态异常) 中发现了 circEx2-6 和 circEx2-7 的降低水平.
circEx2-6表达与精子DFI负相关.
在辅助生殖中,circEx2-7水平与受精率和裂解率相关.

结论:

精子循环RNA,包括circEx3-6,circEx2-6和circEx2-7,显示出男性不孕症的诊断生物标志物的潜力.
这些circRNA与精子运动性,形态学和DNA完整性有关.
环球RNA可能在精子发育和人类生育方面发挥作用,这需要对辅助生殖技术进行进一步的临床研究.

关键词:

辅助生殖技术是一种辅助生殖技术.分裂率是指分裂率是指分裂率.生育率受精率受精率人类环球RNAs.精液参数精液参数精子DNA碎片指数精子DNA碎片指数

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