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相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Next-generation Sequencing03:00

Next-generation Sequencing

88.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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相关实验视频

Updated: Jun 25, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

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Readon:一种新的算法,用于识别长读序列数据的阅读截图.

Siang Chen1,2, Hao Wang1,2, Dongdong Zhang1

  • 1Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Bioinformatics (Oxford, England)
|May 29, 2024
PubMed
概括
此摘要是机器生成的。

科学家们开发了Readon,这是一种使用最小化素描算法的新工具,可以有效地识别从长序列数据中读取的转录. 这推动了对这些重要的RNA分子在发育和疾病中的研究.

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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相关实验视频

Last Updated: Jun 25, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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科学领域:

  • 基因组学就是基因组学.
  • 文字转录学 (Transcriptomics) 是一个学科.
  • 生物信息学是一种生物信息学.

背景情况:

  • 人类基因组中的集群转录活性区域可以导致读透转录,这些转录在发育和瘤发生中起着调节作用.
  • 以前发现读透转录的方法受到下一代测序的短读长度的限制.

研究的目的:

  • 开发一种新的算法,用于准确和快速识别读透转录,特别是从长期和潜在错误的第三代测序数据.

主要方法:

  • 一个最小化素描算法被开发和实施在一个名为Readon.工具中.
  • Readon处理参考序列,识别活跃区域,并使用滑动窗口方法与最小化计算进行索引.
  • 候选阅读截图通过对齐进行选,随后进行确认步骤.

主要成果:

  • 与模拟和真实数据上的现有软件相比,Readon在识别阅读截图方面表现出卓越的性能.
  • 该工具准确而快速地处理长序列数据,克服了以前的局限性.
  • 开发了两个下游工具,用于预测无意中介衰变和可视化拼接模式.

结论:

  • Readon提供了一种有效的解决方案,用于从具有挑战性的测序数据中发现读透转录.
  • 该工具及其相关的下游应用程序有助于进一步研究读透成绩单在生物过程和疾病中的功能作用.