基于智能手机的光学切割 (SOS) 显微镜,具有用于光成像的远程中心设计
Ziao Jiao1,2, Mingliang Pan1, Khadija Yousaf3
1Department of Biomedical Engineering, University of Strathclyde, Glasgow, Scotland, UK.
Journal of microscopy
|May 29, 2024
概括
我们使用HiLo技术开发了一种廉价的智能手机光学切割显微镜. 这个创新的系统提供高对比度成像和光学切割,使先进的显微镜可用于生物医学研究.
科学领域:
- 生物医学光学 生物医学光学
- 显微镜技术 显微镜技术
背景情况:
- 传统的HiLo显微镜系统昂贵而复杂.
- 对各种研究环境而言,需要具有成本效益和便携性的显微镜解决方案.
研究的目的:
- 开发一个基于智能手机的光学切割显微镜 (SOS),利用HiLo技术.
- 为了证明使用智能手机作为照明和检测的核心组件的可行性.
- 以显著降低成本实现高对比度成像和光学切割能力.
主要方法:
- 整合智能手机与现成的光学和机械元件,使用3D打印的适配器.
- 使用智能手机的LED作为光源,与数字镜像设备 (DMD) 相结合.
- 使用电调镜头 (ETL) 在远程中心4f配置中进行低成本的轴向扫描.
主要成果:
- 基于智能手机的光学切割显微镜 (SOS) 实现了571.5微米的远心扫描范围和11.7微米的轴分辨率.
- 成功地对不同波长的光聚烯珠进行了高对比成像.
- 证明了多层光聚烯珠的光学切割.
结论:
- 拟议的基于智能手机的HiLo光学切割显微镜是同类型中的第一个,成本远低于传统系统.
- 这项技术为生物医学研究提供了强大且易于使用的工具,特别是在资源有限的环境中.
- 该SOS系统为需要光学切割的先进成像应用提供了可行的替代方案.
相关概念视频
Confocal Fluorescence Microscopy
13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K
Super-resolution Fluorescence Microscopy
7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Imaging Biological Samples with Optical Microscopy
4.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.7K


