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Updated: Jun 25, 2025

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灵敏的方法分析细胞表面的GPI定蛋白质使用DNA杂交链反应介导信号放大

Sayan Kundu1, Kendall C Craig1, Palak Gupta1

  • 1Department of Chemistry, University of Florida, Gainesville, Florida 32611, United States.

Analytical chemistry
|May 29, 2024
PubMed
概括

研究人员开发了一种新的方法来检测使用代谢工程和DNA信号放大检测甘氨基酸酸氨基醇定蛋白质 (GPI-APs). 这种技术提高了研究这些重要的细胞表面蛋白质的灵敏度.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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