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Updated: Jun 25, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

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Published on: June 11, 2015

菌体冲击蛋白 (PSP) 包裹应激反应:发现新的合作伙伴和进化史

Janani Ravi^1,2, Vivek Anantharaman³, Samuel Zorn Chen⁴

¹Department of Biomedical Informatics, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

|May 29, 2024

概括

细菌菌体冲击蛋白 (PSP) 系统保护细胞免受压力. 我们的研究揭示了它们的古老起源和细菌的多样化进化,揭示了新的组件和功能.

科学领域:

微生物学微生物学
进化生物学进化生物学
结构生物学结构生物学

背景情况:

细菌菌体冲击蛋白 (PSP) 系统对于细胞膜稳定和保护细胞膜应激至关重要,这种应激往往与细菌毒性有关.
对PSP组件的表征是有限的,特别是在蛋白质细菌族之外.

研究的目的:

系统地识别和分析菌体冲击蛋白 (PSP) 同类物,它们的进化模式,域架构和不同细菌系的基因背景.
发现新的功能合作伙伴和PSP系统的进化多样化.

主要方法:

进行比较的基因组学.
蛋白质序列-结构-功能分析.
对PSP同类和域架构的基因分析.

主要成果:

核心PSP蛋白PspA (Snf7) 在最后一个普世共同祖先中存在,表明古代起源.
现在,PSP系统已经在不同的细菌族群中多元化为多个不同的系统.
确定了新的PSP系统合作伙伴,包括Toastrack域,一个HTH相关的信号域-调节器对,以及与ATPase,Cest/Tir-like chaperone和Band-7域的关联.
与SHOCT类域的新型变体的链接表明它们在组装膜相关复合体中的作用.

结论:

PSP系统的古老和多样化的性质凸显了它们在 prokaryotic 应激反应中的基本作用.
PSP系统的模块化为研究细胞外结构和生活方式的谱系特异性适应提供了新的途径.
域架构和基因上下文分析是在比较基因组学中发现保存和新型蛋白质系统的强大工具.

关键词:

在PSP上提供服务.进行比较的基因组学.域名架构域名架构压力反应的信封压力反应.基因组上下文和社区.分子进化分子演变.菌体冲击蛋白反应的反应人类的基因组学.

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