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在Caenorhabditis中lin-12/Notch基因功能的基因重复和进化可塑性

Haimeng Lyu¹, Nicolas D Moya², Erik C Andersen²

¹Department of Molecular Genetics, Ohio State University, 484 W 12th Ave, Columbus, OH 43210, USA.

|May 29, 2024

概括

基因重复可以导致新的功能,但其对发育的影响尚不清楚. 这项研究揭示了线虫中lin-12/Notch基因复制体之间的功能差异,影响了发育网络.

科学领域:

发展生物学发展生物学
进化遗传学进化遗传学
分子生物学分子生物学

背景情况:

基因重复是进化创新的关键驱动力,但重复基因及其调节网络的功能后果仍然在很大程度上未知.
lin-12/Notch信号通路在发育中起着至关重要的作用,了解基因重复如何影响其组件至关重要.

研究的目的:

研究*Caenorhabditis briggsae*中复制的lin-12基因之间的功能区别,并将它们与*Caenorhabditis elegans*中的单副本正基因进行比较.
探索基因复制对发育网络的影响,以及对LIN-12/Notch信号传导的敏感性演变.

主要方法:

基因组序列和基因模型表征以确定*C. briggsae*中的lin-12复制体.
在CRISPR中介的基因组编辑中,在*C. briggsae* lin-12基因中产生功能丧失 (lf) 和功能增加 (gf) 突变.
突变基因的表型分析,以评估功能差异和发育影响.

主要成果:

证实了*C. briggsae*中存在两个完整的lin-12基因,而其他通路基因与*C. elegans*保持一对一的正统性.
功能获取突变显示出功能差异: *Cbr-lin-12.1(gf) *,但不是 *Cbr-lin-12.2(gf) *,导致与 *Cel-lin-12(gf) *类似的发育缺陷.
与*Cel-lin-12(gf) *不同,*Cbr-lin-12.1(gf) *没有表现出主导的表型,并且需要两个突变的等位基因来表现出缺陷,这表明了差异性的补偿能力.

结论:

基因重复可以表现出不同的能力,相互补偿或干扰正常发育.
这项研究揭示了线虫中同时发生的基因重复和对LIN-12/Notch通路活性发育敏感性的演变.

关键词:

口信号传输是没有的.比较的遗传学比较的遗传学基因复制是基因的复制.线虫的阴道发育.

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