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甘ScCAX4是一种对病原体感染耐药性的负调节剂

Chang Zhang^1,2, Zhenxiang Li², Tingting Sun¹

¹National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, Haikou, 571101 Hainan, China.

Journal of agricultural and food chemistry

|May 29, 2024

概括

甘SCCAX4基因通过负面调节疾病抵抗路径,特别是素酸信号通路,增加了植物对真菌病原体的敏感性.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
生物化学生物化学

背景情况:

(Ca2+) 在植物生理过程中起到关键的第二信使作用.
/H+交换器 (CAX) 蛋白质有助于Ca2+运输,但它们在植物疾病抵抗中的作用基本上是未知的.

研究的目的:

描述甘CAX4基因 (ScCAX4) 并研究其在植物疾病耐药性中的功能.
为了确定ScCAX4对*Fusarium solani* var.敏感性的影响. 在Nicotiana benthamiana中发生的 * * 感染.

主要方法:

对*ScCAX4*的序列表征和表达分析.
在Nicotiana benthamiana*中*ScCAX4*的暂时和稳定的遗传转化.
病原体注射和分析植物防御信号通路 (酸,莉酸,乙烯) 和莉酸含量.

主要成果:

在 *N. benthamiana* 中, *ScCAX4* 的过度表达导致了对 *F. solani* 感染的敏感性增加.
*ScCAX4*调节了酸,莉酸 (JA) 和乙烯信号通路中的基因表达.
转基因植物呈现出更高的JA含量,并证实了*ScCAX4*在疾病耐药性方面的负面作用.

结论:

*ScCAX4*通过调节JA信号通路来负面调节植物疾病耐药性.
这项研究提供了CAX基因在植物免疫力中的作用的见解,并为甘分子育种提供了资源.

关键词:

在 CAX 里面,你会看到 CAX.在RNA-seqqq.疾病耐药性疾病耐药性监管机制监管机制甘糖的使用方法

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