一个不涉及CatSper的机制,在内部受精过程中诱导精子向前运动
Sayuri Goto1, Tomoe Takahashi1, Tae Sato1
1Faculty of Science, Biological Division, Yamagata University, Yamagata 990-8560, Japan.
Zoological science
|May 29, 2024
概括
在新中,高的pH值通过可溶性腺酸环酶 (sAC),蛋白激酶A (PKA) 和短暂受体潜能 (TRP) 道触发精子运动,与精子运动启动物质 (SMIS) 合作. 这条通路控制精子尾部波动以向前移动.
科学领域:
- 生殖生物学 生殖生物学
- 精子生理学 精子生理学
- 分子信号传输的方法
背景情况:
- 精子的运动性对于受精至关重要,并且通常由离子通道和交换器 (如CatSper,sNHE和sAC) 调节.
- 一些物种已经失去了这些信号通路的关键组件,需要对替代机制进行研究.
- 内部受精的新,Cynops pyrrhogaster,为研究CatSper独立的精子运动调节提供了一个模型.
研究的目的:
- 调查控制精子尾部波动膜波动的信号通路在Cynops pyrrhogaster中,独立于CatSper.
- 阐明细胞内pH调节和循环AMP在启动精子运动中的作用.
主要方法:
- 用逆转录PCR检测基因表达 (sNHE).
- 精子被暴露在不同的细胞外pH值,蛋提取物,以及特定的抑制剂 (DIDS,KH7,DDA,H89,RN1747) 和激活剂 (二甲基循环AMP).
- 测量了精子细胞质pH值和循环AMP度的变化,并评估了精子运动性 (波浪膜波浪).
主要成果:
- 在新精子中没有检测到sNHE;然而,在高细胞外pH条件下,细胞内pH增加.
- 双碳酸盐运输,可能通过碳酸无水酶和SLC4运输体,以及可溶性腺酸环酶 (sAC) 活性都与提高细胞内pH值和循环AMP水平有关.
- 抑制sAC (KH7),蛋白激酶A (PKA;H89) 和短暂受体潜能 (TRP) 通道 (RN1747) 的抑制剂显著削弱了精子波动,而二碳酸盐则增强了它.
结论:
- 蛋的高pH值启动了一个信号级联,涉及新精子中的sAC,PKA和TRP通道.
- 这条通路与精子运动启动物质 (SMIS) 相互作用,调节波浪膜以获得强烈的前进运动.
- 这些发现突出了这种物种中控制精子运动的CatSper独立机制.
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