这种HOCl干雾对人类细胞是安全的吗?
Rafał Bogdan Lewandowski1, Małgorzata Stępińska1, Łukasz Osuchowski1
1Institute of Optoelectronics, Biomedical Engineering Centre, Military University of Technology, Warsaw, Poland.
PloS one
|May 29, 2024
概括
高度的低酸 (HOCl) 雾化超过300ppm可能会损害人类皮肤细胞. 这项研究在暴露于500ppmHOCl雾的纤维细胞中发现了显著的细胞毒性和遗传毒性.
科学领域:
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
- 消毒技术 消毒技术
背景情况:
- 低酸 (HOCl) 被广泛用作消毒剂.
- 了解HOCl雾的安全性,尤其是在高度下,对于其在各种环境中的应用至关重要.
研究的目的:
- 评估高度HOCl雾化对人类皮肤原发性纤维细胞的细胞毒性和遗传毒性影响.
- 为了确定诱导有害细胞效应的HOCl雾的度值.
主要方法:
- 初级人类皮肤纤维细胞被暴露在300ppm和500ppm度的气溶液HOCl雾中.
- 细胞毒性通过形态变化和细胞死亡分析使用流细胞计进行评估.
- 用中性彗星测定来检测DNA损伤来评估基因毒性.
主要成果:
- 暴露在500ppm的HOCl雾中9分钟,重复四次,导致显著的细胞毒性,包括改变形态和细胞死亡 (49.1%的完整细胞膜).
- 在相同条件下暴露于500ppmHOCl雾的纤维细胞中观察到显著的DNA损伤.
- 当细胞暴露在300ppm的HOCl雾中3分钟时,即使在多次暴露后,也没有观察到显著的细胞毒性或基因毒性影响.
结论:
- 在干雾中以超过300ppm的度喷雾的HOCl可以对人体皮肤纤维细胞产生细胞毒性和遗传毒性作用.
- 这些发现表明,与HOCl雾化消毒相关的度依赖风险.
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