用杀毒性β-propiolactone处理样本并不排除通过大面板亲和蛋白质组进行分析,包括发现生物标志物候选者的可能性
Vanessa M Beutgen1,2, Aditya M Bhagwat1,2, Anna Mary Steitz3
1Institute of Translational Proteomics, Biochemical/Pharmacological Centre, Philipps-Universität Marburg, 35043 Marburg, Germany.
Analytical chemistry
|May 29, 2024
概括
β-propiolactone (BPL) 有效地使病毒失活,但可能会影响蛋白质分析. 这项研究表明,BPL适度影响SomaScan和Olink等亲和蛋白质基平台,保留了大多数差异性蛋白质表达检测.
科学领域:
- 生物化学 生化学
- 蛋白质组学是指蛋白质组学.
- 病毒学 病毒学
背景情况:
- 病毒无活化对于处理传染性样本至关重要.
- β-propiolactone (BPL) 是一种与生物分子反应的杀毒试剂.
- BPL可能会改变蛋白质表位,可能会影响基于亲和性的蛋白质组分析.
研究的目的:
- 评估BPL治疗对蛋白质分析的影响,使用基于aptamer (SomaScan) 和近距离延伸测试 (Olink Target) 平台.
- 评估BPL如何影响血和细胞培养基中差异性蛋白质表达的检测.
- 为了确定亲和蛋白质组学对于分析BPL无活化样本的适用性.
主要方法:
- 来自卵巢癌患者和良性疾病对照者的血样本在BPL治疗后使用SomaScan进行了分析.
- 在BPL治疗后,对T细胞的条件介质进行了Olink Target分析.
- 蛋白质检测变化及其对群体差异的影响在本地和BPL处理样本之间进行了比较.
主要成果:
- BPL 治疗改变了大约三分之一的 SomaScan 测量结果,但大多数相互作用没有受到影响.
- 不到1%的SomaScan目标显示了BPL治疗和疾病状态之间的相互作用效应.
- BPL影响了Olink Target检测到的3.6%的蛋白质,没有可观察到的相互作用效应.
结论:
- BPL治疗对亲和性蛋白质组平台产生中等影响,大多数差异性蛋白质表达信号被保留.
- 高通量亲和蛋白质组学平台适用于分析用BPL无活化的高风险样本.
- 该研究验证了BPL作为一种可行的无活化剂,用于涉及传染物质的蛋白质学研究.
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