在无心形胎盘炎期间对马胎盘微生物群体的表征
Machteld van Heule1, Hossam El-Sheikh Ali2, Hugo Fernando Monteiro3
1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA; Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, 9800, Belgium.
Theriogenology
|May 29, 2024
概括
马中的无心形胎盘炎可能涉及比以前想象的更广泛的细菌. 这项研究在受影响的马胎盘中发现了显著的微生物差异,这表明机会性病原体有助于这种疾病.
科学领域:
- 兽医微生物学 兽医微生物学
- 马类生殖健康 马类生殖健康
背景情况:
- 无心形胎盘炎是一种不清楚病因的马体晚期妊娠疾病.
- 而Crossiella equi和Amycolatopsis spp.则是不同的. 涉及,它们不总是在所有情况下发现,或者存在于健康的胎盘.
研究的目的:
- 为了研究健康的马匹和患有非心形胎盘炎的马匹的马胎盘的微生物组成.
- 为了识别传统认可的罪祸首之外的潜在细菌病原体.
主要方法:
- 使用16S rDNA测序分析了11个健康和22个受影响的马胎盘中的chorioallantois微生物群体.
- 分析包括多样性指数 (Shannon,Chao1) 和β多样性,以比较微生物群落.
主要成果:
- 在非心形胎盘炎样本中,较低的香农指数和较高的Chao1指数表明了失生症.
- 在健康和受影响的胎盘之间观察到β多样性的显著差异 (p < 0.05).
- 在77%的受影响样本中,特定的属 (Amycolatopsis,Crossiella,Lentzea,Mycobacterium,Enterococcus和一个未确定的Pseudonocardiaceae) 主导 (>70%的丰度).
结论:
- 无心形胎盘炎可能涉及比以前认识的更广泛的机会性病原体谱.
- 这些发现表明,马胎盘中的失生状态有助于疾病的病理学.
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