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基于RNA体和铁碳酸信号探针的电化学生物传感器用于C-反应蛋白检测

Zifeng Wang¹, Shuyuan Liu¹, Zhuo Shi¹

¹School of Health Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, 200093, China.

|May 29, 2024

概括

使用Ti3C2Tx MXene和金纳米颗粒的新型电化学生物传感器检测C反应蛋白 (CRP),这是一个关键的炎症生物标志物. 该传感器提供敏感和选择性的CRP检测,可用于诊断感染和心脏病.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
电化学电化学电化学

背景情况:

监测健康生物标志物对于疾病诊断至关重要.
电化学检测C反应蛋白 (CRP) 对于诊断炎症,感染和心脏病非常有价值.

研究的目的:

开发一种新的电化学吸收体生物传感器,用于敏感和选择性CRP检测.
使用Ti3C2Tx MXene和金纳米粒子来提高生物传感器性能.

主要方法:

使用Ti3C2Tx MXene和in-situ降解黄金纳米粒子 (Au NPs) 构建了一种电化学的阿帕特默生物传感器.
用于CRP检测的固定型化RNA体,使用Fc(COOH) 作为信号探针.
通过调整化时间和pH,优化传感器性能.

主要成果:

生物传感器在0.05-80.0 ng/mL的线性范围内显示出CRP检测的高灵敏度.
达到0.026 ng/mL的低检测极限,具有良好的选择性.
在血清样本中成功检测出CRP,恢复率合理.

结论:

开发的电化学阿普坦生物传感器显示了快速轻松检测CRP的巨大潜力.
Ti3C2Tx MXene和Au NPs的组合提高了用于实际诊断应用的生物传感器性能.

关键词:

在C-反应性蛋白质中.电化学生物传感器它们是RNA吸收酶.Ti(3)C(2)T(x) MXene 这种物质是什么?

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