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植物氨酸通过改善抗氧化系统和基因表达,维持了Brassica juncea L.中毒性诱导的氧化突发

Jaspreet Kour¹, Tamanna Bhardwaj¹, Rekha Chouhan²

¹Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, India.

Environmental pollution (Barking, Essex : 1987)

|May 29, 2024

概括

Melatonin (Mel) 通过减少氧化应激和增强抗氧化酶活性,减轻 Brassica juncea 幼苗中的 (Cr) 毒性. 这项研究证明了Mel Mel.

科学领域:

环境科学环境科学
植物生理学植物生理学
生物化学生物化学

背景情况:

土壤中 (Cr) 污染严重影响作物产量,并对人类和植物健康构成风险.
的毒性会诱导植物的氧化应激,其特征是活性氧物种 (ROS) 的增加和细胞组件的损伤.
了解植物对重金属压力的保护机制对于农业的可持续性至关重要.

研究的目的:

调查外源性应用的黑激素 (Mel) 对暴露于 (Cr) 应激的Brassica juncea幼苗的保护作用.
在Cr毒性和Mel治疗下分析B. juncea的形态生理,生化和细胞反应.
阐明Mel在调节抗氧化剂防御系统和减轻Cr诱导的氧化损伤中的作用.

主要方法:

在0.2mM Cr压力下,Brassica juncea苗木接受了不同度的黑素 (0.05,0.1,0.15μM) 的治疗.
测量了形态生理参数,电解质泄漏,以及超氧化物,甲基和过氧化的含量.
分析了抗氧化酶 (SOD,CAT,POD,APOX,GST,DHAR,GPOX,GR) 和基因表达 (RBOH1) 的活性.

主要成果:

的毒性显著增加了氧化应激标志物 (超氧化物,MDA,H2O2) 和电解质泄漏,导致膜和核损伤.
黑色素治疗,特别是0.15μM,有效地减少了ROS生成,抑制了氧化损伤,并保持了膜完整性.
梅尔显著上调抗氧化酶活动和调节基因表达,增强植物在Cr.压力下的生存和发育.

结论:

外源性黑激素为Brassica juncea幼苗提供了重要的保护,防止引起的氧化应激.
黑色素增强了植物的抗氧化防御系统,减少了细胞损伤,从而提高了对重金属毒性的耐受性.
氨酸是减轻农业系统中污染不利影响的有前途的药物.

关键词:

(Chromium) 是一种含的物质.过氧化是一种过氧化.马隆迪阿尔化物黑色素是什么黑色素是什么黑色素是什么有反应性氧物种的反应性氧物种.呼吸系统爆发氧化酶同类物这是一种超氧化离子.

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