在大鼠中,HSP70可保护小鼠免受急性胰腺炎引起的肠壁损伤
Sijin Chen1, Rong Qin1, Zhibo Zhang1
1Department of Gastroenterology, The Affiliated Yan'An Hospital of Kunming Medical University, Kunming 650051, Yunnan, People's Republic of China.
概括
热冲击蛋白70 (HSP70) 在急性胰腺炎 (AP) 期间有助于保护肠道屏障. 在AP大鼠模型中升级HSP70可减少炎症,氧化应激和亡,改善肠道屏障功能.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 手术紧急情况 手术紧急情况
背景情况:
- 急性胰腺炎 (AP) 是一种严重的手术紧急情况,其特征是肠道屏障功能障碍.
- 热冲击蛋白70 (HSP70) 是一种分子伴侣,在AP中具有潜在的治疗益处,但其在肠道屏障完整性中的作用尚不清楚.
研究的目的:
- 在急性胰腺炎的小鼠模型中研究HSP70对肠道屏障功能的影响.
- 阐明HSP70在AP引起的肠损伤中的保护作用背后的分子机制.
主要方法:
- 在老鼠中使用注射的甲酸诱导了急性胰腺炎.
- 使用lentivirus感染来提高HSP70表达的调节.
- 在胰腺和肠道组织上进行了组织学,生物化学,分子和细胞分析.
主要成果:
- AP诱导了显著的胰腺和肠道组织损伤,炎症,氧化应激和屏障破坏,与下调的HSP70表达.
- 提高HSP70的调节改善了组织损伤,减少了炎症标志物,氧化应激 (ROS和mtROS) 和亡.
- 通过恢复紧结蛋白表达,HSP70治疗显著改善了肠道屏障功能.
结论:
- 通过减轻肠道屏障破坏,HSP70在急性胰腺炎中起着保护作用.
- HSP70具有抗炎,抗氧化和抗丧作用,使其成为AP的潜在治疗标.
相关概念视频
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The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
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