由Sox9-Cre指导的Nr5a1的有条件干扰会损害上腺发育
Ayako Tagami1, Yayoi Ikeda2, Kyoko Ishizuka1
1Department of Anatomy, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan.
Scientific reports
|May 29, 2024
概括
在Sox9-表达细胞中Nr5a1的有条件淘汰会破坏上腺发育,导致亡和低成形. 这突显了Nr5a1在上腺原体扩张和器官生成中的关键作用.
科学领域:
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
- 遗传学 遗传学 是一个
背景情况:
- 类固醇原因子1 (Nr5a1) 对上腺的发育和功能至关重要.
- 目前尚不完全了解SOX9在上腺发育中的作用,特别是它与Nr5a1.1的相互作用.
研究的目的:
- 为了研究条件Nr5a1淘汰在Sox9-表达细胞对小鼠上腺发育的影响.
- 为了阐明Nr5a1在早期上腺器官发生过程中的特定功能.
主要方法:
- 使用Sox9-Cre指导的Nr5a1条件淘汰赛小鼠模型 (Sox9-Cre;Nr5a1flox/flox).
- 在各种胚胎阶段 (E10.5-E12.5) 和成年人中分析了上腺体形态,细胞增殖和细胞亡.
- 评估了上腺和类固醇标记物的表达.
主要成果:
- 在早期的上腺皮细胞中,SOX9短暂地表达.
- Sox9-Cre;Nr5a1flox/flox小鼠表现出腺上皮细胞减少和由E12.5.5引起的亡增加.
- 上腺原始扩张,髓形成和封装受损,导致成年上腺的低可塑性,异常的X区分化和真空细胞.
结论:
- Nr5a1对于胎儿上腺皮细胞的存活和增殖至关重要.
- Nr5a1对于上腺原体扩张,正确的髓发育和整体上腺器官生成至关重要.
- 在Sox9-表达细胞中的Nr5a1的有条件切除导致严重的上腺缺血和功能缺陷.
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