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相关概念视频

lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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结核细胞网膜与压力相关的lncRNA预后特征的特征和RP11-295G20.2的瘤抑制作用在肺腺癌中.

Liying Yu1,2,3, Shuang Zhou4, Wencong Hong4

  • 1Central Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China. yly20070567@126.com.

Scientific reports
|May 29, 2024
PubMed
概括
此摘要是机器生成的。

这项研究确定了九种与内质网膜压力相关的长非编码RNA (lncRNAs),可预测肺腺癌 (LUAD) 的预后. 使用这些lncRNAs的风险模型突出了高风险和低风险LUAD患者之间瘤进展和免疫力的差异.

关键词:
细胞内膜网膜应激压力豁免权是一种豁免权.肺部腺癌瘤是肺部腺癌.预测 预后 预后在 RP11-295G20.2 中使用.在 lncRNA 中,

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科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 细胞内膜网膜应激 (ERS) 在瘤微环境中至关重要,通常由蛋白质错误折叠引发.
  • 长非编码RNAs (lncRNAs) 与ERS反应和肺腺癌 (LUAD) 进展有关,但它们在LUAD中的特定预后作用尚不清楚.

研究的目的:

  • 为了确定在LUAD中具有预后价值的ERS相关的lncRNAs.
  • 开发和验证基于ERS相关的lncRNAs的LUAD患者生存预测模型.
  • 研究这些lncRNAs的临床影响和潜在的治疗向.

主要方法:

  • 考克斯和最小绝对收缩和选择操作员 (LASSO) 回归分析被用于识别预后ERS相关的lncRNAs.
  • 构建了一个风险预测模型,将LUAD患者分为高风险 (HS) 和低风险 (LS) 组.
  • 进行了全面的生物信息分析,包括功能丰富和临床样本的验证.

主要成果:

  • 确定了9个与ERS相关的lncRNAs (五个保护性,四个风险因素) 作为独立的预后指标.
  • 开发的风险模型有效预测了整体生存率和分层的LUAD患者.
  • 与LS患者相比,HS患者表现出晚期瘤,更高的突变负担,抗瘤免疫力受损,药物敏感性降低.
  • 功能分析将这些lncRNA与细胞迁移,死亡和免疫联系起来.
  • 降低风险 lncRNA RP11-295G20.2减少了ERS并抑制了LUAD细胞的增殖,入侵和迁移.

结论:

  • 一个与ERS相关的新型lncRNA签名作为一个有价值的生物标志物用于LUAD的预后预测.
  • lncRNA RP11-295G20.2是LUAD的潜在治疗点,因为它的调制会影响ERS和瘤进展.