红色拉斯伯里的高通量色彩测定和色彩参数与总安东素的相关性
Claudia Baldassi1, Clover Lee1, Michael Dossett2
1Wine Research Centre, Faculty of Land and Food Systems, The University of British Columbia, 2205 East Mall, Vancouver, BC, V6T 1Z4, Canada.
Plant methods
|May 29, 2024
概括
使用番茄分析器颜色测试 (TACT) 来测量红色粉的高通量方法的开发对于育种计划至关重要. 与传统的色度计相比,TACT在96孔板上提供了一种更有效的方法来评估拉斯伯里颜色和抗生素含量.
科学领域:
- 园艺园艺 园艺园艺
- 植物育种 植物育种
- 食品科学 食品科学 食品科学
背景情况:
- 红色拉斯伯里果实颜色是消费者首选的一个关键特征,也是全球育种计划的主要目标.
- 评估树颜色的传统方法,如安东素含量分析和色度测量,对于大样本大小来说是耗时的.
- 开发高通量方法对于草育种中有效选至关重要.
研究的目的:
- 开发一种高吞吐量方法来测量红色拉斯伯雷泥的颜色.
- 为了评估颜色参数和红色树中总安东素含量之间的相关性.
- 为了比较色度计与番茄分析器颜色测试 (TACT) 的有效性,用于树颜色评估.
主要方法:
- 551个红色拉斯伯里泥样品的颜色坐标 (L*,a*,b*) 用色度计和番茄分析仪颜色测试 (TACT) 来测量.
- 在两个培养皿和96井盘中的样品中使用了TACT.
- 从所有样本中提取了总的安托西亚宁,用于内容分析.
主要成果:
- 颜色参数 (L*,a*,b*) 的回归模型显示,在培养皿和96孔板 (R2 ≥0.93) 中的TACT测量之间存在很高的一致性.
- 通过TACT测量的颜色参数和总抗生素含量之间的相关性是显著的和中等强的 (例如,R = -0.69对于L*).
- 使用传统色度计测量的颜色参数与TACT相比,与总安东素的相关性较弱.
结论:
- 番茄分析器色彩测试 (TACT),特别是使用96孔板,提供了一种比传统色度仪更有效的高吞吐量方法来测量红树质粉色.
- 虽然TACT更好地捕捉了大基因型之间的颜色差异,但与总安东素的相关性对于直接预测模型来说还不够强.
- 使用96井板的TACT方法显著提高了在育种计划中调色彩分析的吞吐量.
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