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免疫细胞亚群的动态变化在脂肪组织的热能激活中

Yuqing Ye¹, Huiying Wang¹, Wei Chen¹

¹National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.

Frontiers in immunology

|May 30, 2024

概括

暴露于寒冷会改变热生脂肪组织中的免疫细胞群. 单细胞和T细胞增加,而NK细胞减少,影响能量消耗和代谢健康.

科学领域:

免疫学免疫学免疫学
代谢健康代谢健康
脂肪组织生物学脂肪组织生物学

背景情况:

暴露于寒冷影响全身新陈代谢,棕色和色脂肪组织在寒冷诱导的热生成中发挥着关键作用.
这些热生脂肪组织对于能量消耗至关重要,并有可能对抗代谢障碍.
虽然热原脂肪组织的免疫调节是公认的,但不同寒冷时期免疫细胞的明显变化尚未得到充分理解.

研究的目的:

为了研究不同寒冷暴露持续时间下的热生脂肪组织内免疫细胞概况的整体变化.
为了确定在寒冷挑战期间参与脂肪组织热能调节的特定免疫细胞亚群.

主要方法:

在不同的寒冷时期,使用Cibersort和mMCP-counter算法对棕色和色脂肪组织进行免疫透分析.
使用单细胞测序数据集 (GSE207706) 验证免疫细胞群变化.
通过流式细胞计,免疫光学和实时定量PCR在小鼠模型中的实验验证,以评估免疫细胞比例和表达.

主要成果:

寒冷暴露导致棕色脂肪组织中单细胞和原始和记忆T细胞的比例增加.
在寒冷条件下,在棕色脂肪组织中观察到自然杀手 (NK) 细胞的比例下降.
在发热脂肪组织中发现了免疫细胞概况的动态变化.

结论:

这项研究揭示了热源性脂肪组织内免疫细胞组成的动态变化,以应对寒冷暴露.
确定了新的免疫细胞亚群,可能有助于脂肪组织在寒冷期间的热能激活.
这些发现增强了对免疫系统和热生成在代谢调节中的相互作用的理解.

关键词:

脂肪组织脂肪组织.生物信息学是一种生物信息学.暴露在寒冷中的时间.免疫细胞免疫细胞热生成是一种热生成.

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