在典型的输注速率下输注多巴胺不会干扰血肌素测定
Jenny Yeuk Ki Cheng1,2, Shreenidhi Ranganatha Subramaniam1,2, Stephanie C Y Yu1,2
1Department of Chemical Pathology, Prince of Wales Hospital, Hospital Authority, Hong Kong.
多巴胺输液不会干扰肌素测试. 然而,多巴胺污染可能会对酶类肌氨酸测定产生负面影响,而贾菲测定则显示干扰较少. 使用Jaffe测试来交叉检查是否怀疑多巴胺干扰.
科学领域:
- 临床化学 临床化学
- 生物化学 生化学
- 分析化学 分析化学
背景情况:
- 众所周知,多巴胺会干扰酶性肌氨酸测量.
- 多巴胺对雅菲反应的具体影响以及在酶测定中引起干扰的度尚未明确.
研究的目的:
- 为了研究稳定多巴胺输注对雅菲和酶性肌氨酸试验的干扰.
- 为了评估多巴胺输注滴滴臂污染对这些肌氨酸测试的影响.
主要方法:
- 多巴胺被添加到血样本中的度模拟输液速率 (2-20μg/kg/min).
- 模仿临床制剂的多巴胺溶液与血混合,以模拟滴水臂的污染.
- 使用Jaffe和酶方法测量肌氨酸水平.
主要成果:
- 高达20μg/kg/min的多巴胺输液并没有干扰肌素测量.
- 多巴胺污染显著干扰了酶性肌氨酸测定,即使在低水平.
- 贾菲反应显示多巴胺污染的干扰程度较小.
结论:
- 氨酸可以使用Jaffe或酶方法从没有多巴胺污染的地点抽取的样本中可靠地测量.
- 在怀疑多巴胺干扰时,贾菲反应可以作为酶分析的有用交叉检查.
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