在胆结石和胆囊癌之间识别长非编码RNA表达特征,使用下一代测序分析
Qiang Wang1, Pinduan Bi1, Ding Luo1
1Department of Hepatobiliary Surgery, the First Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.
International journal of general medicine
|May 30, 2024
概括
胆结石病 (GS) 可以导致胆囊癌 (GBC),但机制尚不清楚. 这项研究确定了参与GS促进的GBC进展的长非编码RNA (lncRNA) 和信使RNA (mRNA),提供了潜在的诊断和治疗标.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 胆结石病 (GS) 是胆囊癌 (GBC) 的重要危险因素.
- 将GS与GBC进展联系起来的精确分子机制在很大程度上是未知的.
- 长非编码RNAs (lncRNAs) 正在成为包括癌症在内的各种疾病的关键调节者.
研究的目的:
- 确定在胆结石疾病和胆囊癌中差异表达的lncRNA和信使RNA (mRNA).
- 为了阐明可能与胆结石促进的胆囊癌进展有关的分子途径.
- 发现早期诊断和针对性治疗GBC的潜在分子特征.
主要方法:
- 对四组进行RNA测序:没有GS的GBC患者,正常的胆囊组织,胆结石和GS的GBC患者.
- 对差异表达 (DE) lncRNA和mRNA进行分析,以确定重叠和特定的分子特征.
- 在关键信号通路中对共同差异表达基因 (co-DEGs) 和 lncRNAs (co-DElncRNAs) 的功能丰富分析.
- 使用qRT-PCR对关键基因 (FXYD2,MPZL1,PAH) 的验证.
主要成果:
- 共有29个共同DEG和500个共同DElncRNA被确定,与胆结石或GBC相关.
- 涉及GBC发育的关键途径包括焦点粘附,癌症中的转录失调,蛋白质消化和吸收以及ECM受体相互作用.
- 胆结石的转录形状更像是正常的胆囊组织而不是GBC,这表明癌症风险较低.
- 在共同DEG和共同DElncRNA中发现并验证了特定基因 (FXYD2,MPZL1,PAH).
结论:
- 这项研究确定了一系列不同表达的基因和参与GBC和GS相关代谢途径的lncRNAs.
- 这些发现表明,与胆结石相关的转录基因变化与GBC不同,表明潜在的风险概况较低.
- 对这些分子特征的进一步调查对于推进GBC的早期诊断和治疗策略至关重要.
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