内皮细胞衍生纤维素和血纤维素在内皮功能障碍中的不同作用
Xiaoxin Luo1, Weixiong Jian1,2
1Department of Traditional Chinese Medicine Diagnostics, Faculty of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
Turkish journal of medical sciences
|May 30, 2024
概括
内皮纤维素 (FnEC) 促进动脉样硬化,而血纤维素 (pFn) 抑制了它. 这项研究区分了它们在内皮细胞激活和功能障碍中的作用.
科学领域:
- 心血管生物学 心血管生物学
- 细胞和分子医学 细胞和分子医学
- 动脉样硬化研究 动脉样硬化研究
背景情况:
- 纤维素 (Fn) 存在于动脉样硬化斑块中,影响内皮炎症和激活.
- 细胞Fn (cFn) 和血Fn (pFn) 是不同的形式,具有不同的结构和功能.
- 不同Fn类型在内皮功能障碍中的具体作用尚不清楚.
研究的目的:
- 调查血纤维素 (pFn) 和内皮细胞衍生纤维素 (FnEC) 在内皮细胞激活和功能障碍中的不同作用.
- 阐明这些纤维素蛋白变体影响动脉样硬化发展的机制.
主要方法:
- 用氧化低密度脂蛋白 (ox-LDL) 诱导内皮细胞损伤.
- 产生了一种突变的甲状腺内皮细胞菌株,具有纤维内素敲除 (FnEC-KD).
- 对比了FnEC-KD和pFn添加对炎症标志物和内皮功能的影响.
主要成果:
- FnEC-KD显著抑制了ox-LDL诱导的ICAM-1,VCAM-1,内甲蛋白和NFκB激活的表达,表明内皮激活和功能障碍的抑制.
- 相反,pFn添加显著抑制了ox-LDL诱导的ICAM-1,VCAM-1,内甲蛋白表达和NFκB激活.
- 氧-LDL刺激导致过度的氧化生产,加剧了内皮细胞损伤.
结论:
- 大动脉内皮细胞衍生的纤维素 (FnEC) 促进内皮细胞的激活和功能障碍.
- 血纤维菌素 (pFn) 抑制ox-LDL诱导的内皮细胞激活和功能障碍,这表明它具有保护作用.
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