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Updated: Jun 25, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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转录因子结合部位的发育和进化特征基于可解释的机器学习模型集群区域

Zhangyi Ouyang1, Feng Liu2, Wanying Li1

  • 1Academy of Military Medical Sciences, Beijing 100850, China.

Nucleic acids research
|May 30, 2024
PubMed
概括

这项研究揭示了转录因子结合部位在物种和发育之间如何演变. 了解这些 cis 调节元素中的动态是解释表型变异的关键.

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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