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Updated: Jun 25, 2025

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在子宫内膜癌中,N6-Methyladenosine修饰的circ-NAB1通过CDKN3调节细胞周期和上皮-介质细胞过渡

Jun Zhang¹, Yang Zhang², Jinwei Zhang³

¹Department of Gynecology, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi, Jiangsu 214023, China. viola0116@163.com.

Cellular and molecular biology (Noisy-le-Grand, France)

|May 30, 2024

概括

循环RNANAB1 (circ-NAB1) 通过影响细胞增殖和迁移,促进子宫内膜癌 (EC) 的进展. 它的表达受到m6A修饰的调节,影响EC细胞中的miR-876-3p/CDKN3轴.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

子宫内膜癌 (EC) 是一种流行的妇科恶性瘤.
循环RNAs (circRNAs) 和N6-甲基氨酸 (m6A) 修饰与癌症的发展有关.
目前尚不清楚circ-NAB1的具体作用及其在EC中与m6A的相互作用.

研究的目的:

为了研究circ-NAB1在子宫内膜癌中的功能.
探索EC中circ-NAB1和m6A修饰之间的交叉对话.
为了阐明circ-NAB1在EC进展中的潜在分子机制.

主要方法:

定量实时PCR (RT-qPCR) 用于测量circ-NAB1的表达.
进行MeRIP和RNA拉下测试以检测m6A修饰和RNA相互作用.
细胞增殖,迁移,入侵,细胞循环和上皮细胞-介质细胞转换 (EMT) 试验.

主要成果:

在EC组织和细胞中,Circ-NAB1被上调.
ALKBH5通过一个m6A-YTHDF2-依赖的通路调解circ-NAB1表达.
过度表达Circ-NAB1促进了EC细胞的增殖,迁移,入侵,EMT和细胞循环.
在Circ-NAB1的海绵式miR-876-3p上调调节CDKN3,从而扭转circ-NAB1耗尽效应.

结论:

对circ-NAB1的ALKBH5-介导的m6A修饰促进了EC中的EMT和细胞循环.
环-NAB1/miR-876-3p/CDKN3轴是子宫内膜癌进展的关键途径.

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