最近的新增以及访问包含液体染色学,离子流动性光谱学和双重质谱学信息的多维脂质学数据库
Amie M Solosky1, Kaylie I Kirkwood-Donelson2, Melanie T Odenkirk3
1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Analytical and bioanalytical chemistry
|May 30, 2024
概括
研究人员开发了一个新的多维脂质数据库,改善了它们的识别和分离. 该资源通过提供关键的分析数据,有助于理解脂质在疾病和环境因素中的作用.
科学领域:
- 脂管学和分析化学 分析化学
- 生物分子分析和数据科学
背景情况:
- 脂质越来越多地与疾病,微生物组变化和环境暴露有关,需要先进的分析方法.
- 由于化学成分多样化,脂质组分析具有挑战性,需要专门的提取和分离技术来分离异构体和异构体.
研究的目的:
- 呈现一个多维的脂质数据库,以提高脂质物种的分离和识别.
- 为各种生物流体和组织中877种独特的脂质提供实验数据,扩大之前的工作.
主要方法:
- 利用一个平台,结合逆相液态色谱,漂流管离子运动谱,碰撞诱导解离和质谱 (RPLC-DTIMS-CID-MS).
- 收集了关于多重脂质前体离子的保留时间,IMS碰撞截面 (CCS) 值,m/z信息和CID碎片的数据.
- 在5个脂质类别和24个脂质类别中组织数据库.
主要成果:
- 创建了一个全面的数据库,包含实验RPLC保留时间,IMS CCS值和877种独特脂质的m/z数据.
- 包括1504种前体离子物种和相关的CID碎片,与临床和环境研究相关.
- 与传统方法相比,多维RPLC-DTIMS-CID-MS平台可以实现更有信心的脂质注释.
结论:
- 开发的多维数据库显著改善了脂质识别和注释,特别是对异构脂质.
- 该资源有助于研究人员研究脂质与疾病,微生物组和环境因素的关联.
- 已经创建了一个可访问的网页,以定期更新和共享这个有价值的脂质学数据资源.
关键词:
碰撞的横截面截面是什么数据库数据库数据库是一个数据库.离子移动性光谱仪离子移动性光谱仪利皮多米克 (Lipidomics) 是一种消化剂.脂质 脂质 脂质 是一种质谱测量质量谱测量逆相液态色谱 (RPLC) 是一种反相液态色谱.更多相关视频
08:56Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
7.3K
09:00Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
Published on: July 14, 2022
3.2K
相关概念视频
01:19MALDI-TOF Mass Spectrometry
4.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.8K
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K