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罗素通过多种机制抑制传染性胃肠炎病毒的扩散

Yuting Duan^1,2,3, Haichuan Li³, Shuai Huang⁴

¹State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.

The Journal of general virology

|May 30, 2024

概括

罗素通过向病毒的进入和复制,有效地抑制传染性胃肠炎病毒 (TGEV) 的扩散. 这种天然化合物显示出作为治疗TGEV和其他冠状病毒感染的治疗剂的希望.

科学领域:

兽医病毒学兽医病毒学
药物发现药物发现药物发现
分子生物学分子生物学

背景情况:

传染性胃肠炎病毒 (TGEV) 在猪业中造成重大经济损失,原因是猪死亡率高,肠道受损.
目前对TGEV的治疗选择有限,需要开发新的抗病毒药物.

研究的目的:

识别和描述针对TGEV的新型治疗剂.
为了研究弗洛雷丁对TGEV感染的抗病毒机制.

主要方法:

在体外抗病毒试验中,使用感染TGEV的PK-15细胞进行了试验.
病毒基因表达 (N蛋白,mRNA) 和病毒标位的分析.
网络药理学和分子对接以确定潜在的药物点.
产生和分析耐佛洛素的TGEV菌株.

主要成果:

罗素证明了TGEV扩散的剂量依赖性抑制,减少病毒N蛋白,mRNA和标位.
罗的抗病毒活性针对TGEV内部化和复制阶段.
罗素降低了TGEV诱导的炎症性细胞因子表达.
网络药理学确定了潜在的目标,包括AR,CDK2和MAPK14.
抗性突变映射到主要蛋白酶 (Mpro) S242残留物,表明Mpro是关键目标.

结论:

罗是一种强大的TGEV抑制剂,其机制涉及病毒进入和复制.
罗通过减少促炎性细胞因子,表现出免疫调节作用.
该研究将Mpro确定为弗洛雷丁抗TGEV活性的潜在目标.
罗代表了治疗TGEV和潜在的其他冠状病毒感染的有希望的候选药物.

关键词:

这是一种抗病毒药物.炎症因素是炎症因素.佛罗里丁是一种色素.复制复制复制复制复制复制复制传染性胃肠炎病毒 (TGEV)

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