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Updated: Jun 25, 2025

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生物活性蛋白的单步净化和N-终端功能化通过非典型的分裂整体

Ryan Gharios1, Annabella Li1, Irina Kopyeva2

  • 1Department of Chemical Engineering, University of Washington, Seattle, Washington 98105, United States.

Bioconjugate chemistry
|May 30, 2024
PubMed
概括

我们开发了VidaL标记表达和蛋白质结合 (VEPL) 以实现高效的N端蛋白质修饰和净化. 这种方法可以实现功能性,N端修饰蛋白质的模块化和可扩展的生产,具有原生生物活性.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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