解开复杂的联系:在麦固态发酵中,挥发性化合物,自由氨基酸和代谢物的相互作用
Juan Sun1, Waleed Al-Ansi2, Lamei Xue1
1State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
这项研究揭示了用特定的微生物发酵麦如何改变其风味化合物. 关键的挥发性化合物和代谢物发生变化,在发酵麦中形成了与未发酵麦相比的独特形状.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物化学 生化学
背景情况:
- 麦发酵涉及复杂的生物化学转化.
- 微生物活动显著影响麦的感官特性.
- 了解这些变化对于开发基于麦的新产品至关重要.
研究的目的:
- 为了研究在麦发酵过程中挥发性化合物,自由氨基酸和代谢物的动态变化.
- 为了确定Saccharomyces cerevisiae和Lactobacillus plantarum对麦成分的特定微生物影响.
- 阐明在发酵麦中形成风味的基础的生物化学机制.
主要方法:
- 气色谱-质谱 (GC-MS) 用于挥发性化合物分析.
- 正角 - 部分最小平方差分分析 (OPLS-DA) 用于配置差异化.
- 代谢物查和相关性分析,以链接化合物和代谢物.
主要成果:
- 挥发性化合物的显著变化,增加了赫和酒精,减少了化物和酸.
- 在发酵后出现了4种新的化合物.
- 大多数自由氨基酸的减少,血清蛋白显著增加.
- 鉴定了354种不同的代谢物,包括异氧和乳酸伊米达.
- 不发酵 (NFO) 和发酵麦 (FO) 之间明显的挥发性概况,由OPLS-DA证实.
结论:
- 由Saccharomyces cerevisiae和Lactobacillus plantarum进行的微生物发酵极大地改变了麦的挥发性和代谢特征.
- 这些变化是发酵麦独特的风味特征的原因.
- 相关性分析突出了影响麦风味调节的复杂生化途径.
更多相关视频
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
相关概念视频
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Phase I Reactions: Oxidation of Carbon-Heteroatom and Miscellaneous Systems
In carbon-nitrogen systems, aliphatic and aromatic amines can undergo oxidative reactions. Secondary and tertiary amines, like those found in tricyclic antidepressants, can undergo N-dealkylation, a process that involves the oxidation of the alkyl group. In addition, oxidative...
Phase II Reactions: Sulfation and Conjugation with α-Amino Acids
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
