一种多功能 evanescent 波生物传感器,用于对 SARS-CoV-2 变体的普遍测定和对冠状病毒尖端蛋白-hACE2 相互作用的亲和力分析
Bo Jiang1, Yi Yang2, Rongtao Zhao2
1Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China.
Biosensors & bioelectronics
|May 30, 2024
概括
一个新的生物传感器,My-SPACE,在10分钟内快速检测到SARS-CoV-2变种. 这种光纤设备提供了一种灵敏,具有成本效益的工具,用于早期警告冠状病毒传播和评估新出现的传染病.
科学领域:
- 生物医学工程 生物医学工程
- 传染病诊断 诊断 传染病诊断
- 生物传感技术的技术
背景情况:
- 传统的病原体检测方法与新出现的传染病和频繁的突变作斗争.
- 冠状病毒的尖端蛋白突变增强了与人类ACE2的结合,影响了传播和免疫逃避.
研究的目的:
- 开发一种通用生物传感器,用于快速检测冠状病毒和尖端蛋白-hACE2亲和力分析.
- 为新出现的冠状病毒创建一个具有成本效益的现场检测工具.
主要方法:
- 开发一个多功能光纤虚波生物传感器 (My-SPACE).
- 利用与Cy5.5-hACE2的竞争性结合用于自动检测SARS-CoV-2和变种.
- 使用表面等离子共振 (SPR) 验证的亲和度测量.
主要成果:
- 在10分钟内,My-SPACE检测到SARS-CoV-2和变种,在灵敏度和速度上超过了ELISA.
- 在临床样本中实现了对Omicron变异的100%特异性和94.10%敏感性.
- 准确地描述了各种冠状病毒突变的尖端蛋白-hACE2相互作用.
结论:
- 我的空间提供了一个快速,灵敏和可重复使用的平台,用于冠状病毒检测和风险评估.
- 允许初步评估新出现的冠状病毒的传染性和危险性.
- 提供了一个具有成本效益的解决方案,用于在现场预警冠状病毒传播风险.
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