美国东南部的Cyclospora cayetanensis的来源和流行情况. 生长环境的生长环境
Amy M Kahler1, Jessica Hofstetter2, Michael Arrowood1
1Centers for Disease Control and Prevention (CDC), Division of Foodborne, Waterborne, and Environmental Diseases, Atlanta, GA 30329, USA.
Journal of food protection
|May 30, 2024
概括
在格鲁吉亚没有检测到使用qPCR的Cyclospora cayetanensis (C. cayetanensis) 生产环境. 错误的阳性结果很常见,突出了对这种寄生虫的环境测试中基于序列的确认的需要.
科学领域:
- 环境微生物学环境微生物学
- 食品安全 食品安全
- 寄生虫学的寄生虫学
背景情况:
- 与美国农产品相关的环状病爆发需要了解C. cayetanensis (C. cayetanensis) 在农业环境中的流行情况.
- 之前的研究表明,农业环境中的潜在污染途径.
研究的目的:
- 评估来自格鲁吉亚东南部农业地区的各种环境样本中C. cayetanensis的发生情况.
- 评估qPCR方法在环境矩阵中检测C. cayetanensis的可靠性.
主要方法:
- 在两年内收集了市政废水污泥,农场便携式所,灌池水和废弃包装房屋垃圾桶水的样本.
- 使用定量聚合酶连锁反应 (qPCR) 针对C. cayetanensis 18S rRNA基因.
- 测序和人类特异性便标记物 (HF183,crAssphage) 用于确认和评估便污染.
主要成果:
- 通过测序证实了没有C. cayetanensis qPCR检测,表明可能由于测试交叉反应性而导致的显著错误阳性.
- 在9%的废水污泥样本中发现了确认的C. cayetanensis qPCR检测.
- 在池和垃圾桶样本中检测到人类便标记物,但与C. cayetanensis qPCR信号没有发现相关性.
结论:
- 在使用序列确认的qPCR时,该研究在评估的农业环境样本中没有发现C. cayetanensis的证据.
- 测试特异性和严格的检测标准,包括基于序列的确认,对于准确的C. cayetanensis的环境检测至关重要.
- 建议使用基于序列的方法进行进一步的研究,以可靠地解释C. cayetanensis的环境qPCR数据.
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