相关实验视频

Updated: Jun 25, 2025

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines

Published on: January 19, 2024

分子工程提高反应性氧物种的产生,以对脂质滴和线粒体造成协同损害

Mingdi Yang¹, Kaiwen Li², Liangchen Zhong¹

¹Anhui Key Laboratory of Advanced Building Materials, School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei, 230601, PR China.

Analytica chimica acta

|May 30, 2024

概括

新的光敏化剂 (PSs) 有效地向癌细胞. CIPh证明了脂质滴和线粒体的双重向,通过产生活性氧物种 (ROS) 来诱导亡来增强光动力疗法 (PDT).

科学领域:

生物化学生物化学
材料科学材料科学材料科学
在瘤学瘤学.

背景情况:

光动力疗法 (PDT) 是一种有前途的癌症治疗方法,因为它的高效率和最小的副作用.
在PDT中的挑战包括有限的活性氧物种 (ROS) 扩散和缺氧瘤环境.
开发具有子器官向性和低氧依赖性的光敏化剂 (PSs) 对于有效的PDT至关重要.

研究的目的:

为增强光动力学疗法设计和合成新型光敏化剂 (PSs).
研究设计PSs的亚细胞准能力和ROS生成.
评估PSs在诱导癌细胞亡中的治疗疗效.

主要方法:

基于理论计算的三个PS (CIH,CIBr,CIPh) 的分子工程.
使用理论计算评估S1-T1的能量差距 (ΔE_S1-T1).
评估CIPh.的双重亚细胞向 (脂质滴和线粒体) 和ROS生成 (单氧氧和超氧化离子激素).
在HepG2细胞中诱导亡的评估.

主要成果:

由于其脂性和电阳性,CIPh表现出对脂滴和线粒体的双重向.
在光照射后,CIPh证明了单片氧 (¹O₂) 和超氧化离子基 (O₂^⦁-) 的有效生成.
CIPh治疗导致脂质滴和线粒体膜潜力的完整性受到损害.
在用CIPh治疗的HepG2细胞中诱导了深度亡.

结论:

通过分子工程设计的CIPh显示出作为光动力学疗法的有效光敏剂的潜力.
双重准和高效的ROS生成有助于CIPh在诱导癌细胞亡方面的有效性.
这项研究为开发用于光成像引导的低氧瘤治疗的先进PS提供了基础.

关键词:

死亡机制的死亡机制双细胞下细胞器官准目标分子工程是分子工程.第I类和第二类的PSS.

更多相关视频

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

相关概念视频

Radical Autoxidation

Radical Autoxidation

The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Peroxisomes

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.

Peroxisomes and Mitochondria

Peroxisomes and Mitochondria

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...