经过基因工程改造的Lactococcus lactis菌株构成性地表达GABA产生基因,并产生高水平的GABA
Marcos P Monteiro1, Hannah M Kohl2, Jean-Baptiste Roullet3
1Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, RRC 3070, Atlanta, GA 30322, United States.
Letters in applied microbiology
|May 30, 2024
概括
改造的乳球球菌 (Lactococcus lactis) 产生胺黄油酸 (GABA) 的组成部分. 这种增强GABA合成的益生菌菌株对治疗与低GABA水平相关的疾病具有前景.
科学领域:
- 微生物学 微生物学
- 神经科学是一个神经科学.
- 生物技术是生物技术.
背景情况:
- 胺黄油酸 (GABA) 是一种重要的抑制性神经递质,影响神经健康.
- 降低GABA水平与抑郁症和多发性硬化症等疾病有关.
- 益生菌细菌为治疗性GABA生产提供了一个潜在的途径,但目前的方法很复杂.
研究的目的:
- 为构成GABA合成设计乳球菌,绕过外源诱导剂的需要.
- 为了增强L. lactis中的GABA生产,使用强大的构成性促进剂和GABA合成基因.
主要方法:
- 使用构成性促进体 (P2,P5,P8s) 将GABA合成 (gadB) 和运输 (gadC) 基因克隆到L. lactis中.
- 工程塑体的转化为L. lactis.
- 量化gadCB基因表达和GABA合成,有或没有谷氨酸补充.
主要成果:
- 改造的L. lactis菌株表现出显著增加的gadCB表达 (与P8s相比高达627倍).
- 菌株L. lactis-P8s-GAD表现出显著更高的GABA合成,特别是在补充谷氨酸时.
- 与未经修改的菌株相比,P8s中的表达水平显著更高 (P=0.0325).
结论:
- 在L. lactis中,GABA合成基因的构成性表达是可行的,并增强GABA生产.
- 改造的L. lactis-P8s-GAD菌株代表了与低GABA相关的疾病治疗应用的有希望的候选人.
- 这种方法简化了基于益生菌的GABA疗法的开发.
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