在本地组装状态下植物光系统I的结构将PsaF定义为监管检查点
Andreas Naschberger1, Mariia Fadeeva2, Daniel Klaiman2
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
Nature plants
|May 30, 2024
概括
研究人员发现了一种植物光系统I (PSI) 组装中间体缺少关键子单位,包括PsaF和LHCI. 这一发现揭示了PsaF.
科学领域:
- 植物分子生物学 植物分子生物学
- 光合作用研究研究光合作用.
- 蛋白质复合体组装组件
背景情况:
- 植物光系统I (PSI) 是一个关键的阳光驱动的氧化还原酶复合体.
- PSI包括许多核和塑编码的子单元,这些子单元对其功能至关重要.
研究的目的:
- 阐明PSI组装中间体的结构和功能特性.
- 确定参与PSI生物发生的关键监管子单位.
主要方法:
- 从绿化麦苗木中隔离PSI组装中间体.
- 介质的结构分析以确定子单位组成.
主要成果:
- 隔离的中间体缺少至少八个子单位,特别是PsaF和光收获复合物I (LHCI).
- 该中间体没有光还原活性,表明组装不完整.
- PsaF被确定为LHCI组装的关键监管检查点.
结论:
- PsaF子单元在调节LHCI组装方面发挥着关键作用.
- 该研究通过PsaF检查点直接将PSI生物发生与其功能能力联系起来.
- 了解PSI组装是优化光合作用效率的关键.
更多相关视频
08:40Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
Published on: February 14, 2019
8.3K
13:52Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy
Published on: June 23, 2016
12.2K
相关概念视频
Photosystem I
62.0K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
62.0K
Photosystems
4.8K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
4.8K
Photosystem II
70.1K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
70.1K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
The Photochemical Reaction Center
4.1K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.1K
The Cell Cycle Control System
2.8K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
2.8K
