Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Hematopoiesis01:21

Hematopoiesis

5.2K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.2K
Production of Formed Elements01:34

Production of Formed Elements

1.4K
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
1.4K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

3.9K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
3.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dual CDK2 and CDK4/6 inhibition suppresses Rb/E2F signaling and enhances anti-leukemic activity in acute myeloid leukemia.

Haematologica·2026
Same author

Mendelian susceptibility to mycobacterial disease: Clinical, immunological, and genetic study in India.

Journal of human immunity·2026
Same author

Proximity-Induced Rewiring of Oncogenic Kinase Triggers Apoptosis.

ACS central science·2026
Same author

Erratum: Dual CARM1-and IKZF3-targeting: A novel approach to multiple myeloma therapy.

Molecular therapy. Oncology·2025
Same author

Dual CARM1-and IKZF3-targeting: A novel approach to multiple myeloma therapy synergy between CARM1 inhibition and IMiDs.

Molecular therapy. Oncology·2025
Same author

Dual therapeutic targeting of MYC and JUNB transcriptional programs for enhanced anti-myeloma activity.

Blood cancer journal·2024

相关实验视频

Updated: Jun 25, 2025

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.4K

BRD9 调节正常的人类造血干细胞功能和血统分化.

Swati Garg1,2, Wei Ni1,2, Basudev Chowdhury1,2

  • 1Dana-Farber Cancer Institute, Dept. of Medical Oncology, Boston, MA, 02215, USA.

Cell death and differentiation
|May 30, 2024
PubMed
概括

含原体的蛋白9 (BRD9) 对于人类正常的血细胞发育至关重要. 抑制BRD9会损害造血干细胞的功能和分化,突显其在表观遗传调节中的作用.

更多相关视频

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.5K
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

11.0K

相关实验视频

Last Updated: Jun 25, 2025

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.4K
Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.5K
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

11.0K

科学领域:

  • 血液学 血液学 血液学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 染色体生物学 染色体生物学

背景情况:

  • 含原蛋白9 (BRD9) 是非正规的BRG1/BRM关联因子 (ncBAF) 染色体重塑复合物的组成部分.
  • BRD9是急性髓性白血病 (AML) 中已知的合成致命标.
  • 在正常人血液形成中BRD9的功能仍然在很大程度上是未知的.

研究的目的:

  • 调查BRD9在正常人类造血干细胞和原生细胞 (HSPC) 功能中的作用.
  • 阐明BRD9影响血液形成的分子机制.

主要方法:

  • 人类HSPC中BRD9的基因组和化学抑制.
  • 在体外殖民地形成试验 (GEMM殖民地).
  • 血统特定的差异化文化.
  • 异种移植试验.异种移植试验.
  • RNA测序 (RNAseq) 和染色体可访问性分析.
  • 在 GATA1-luciferase 记者测试中.

主要成果:

  • 在HSPC中抑制BRD9导致干细胞的增殖和损失减少.
  • 降低的BRD9水平损害了未成熟的混合多能GEMM殖民地的形成.
  • BRD9的淘汰导致了巨核细胞分化失败,延迟了红状腺分化,并增强了髓状腺分化.
  • 有BRD9倒置的HSPCs在体内显示长期多系植入减少.
  • RNAseq在BRD9敲击后揭示了下调的基因和抑制转录因子促进者的增强色素可访问性.
  • 由造血主调节器GATA1调节的BRD9损失调节基因网络,与BRD9抑制减少GATA1活动.

结论:

  • 在人类HSC分化过程中,BRD9在维持活跃的转录中起着至关重要的作用.
  • BRD9对于适当的造血干细胞功能和多系分化至关重要.
  • BRD9通过表观遗传调节影响血液形成,影响关键的转录因子,如GATA1.1.