该PRR-EC复合体和SWR1染色体重塑复合体的功能合作,通过调节PIF4表达在Arabidopsis中抑制夜间阴囊延长
Jin Hoon Won1, Jeonghyang Park2, Hong Gil Lee3
1Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
Plant communications
|May 31, 2024
概括
植物生理时钟组件PRR5,TOC1和ELF3相互作用,通过染色体重塑抑制PIF4表达和夜间阴囊延长. 这种表观遗传机制确保了Arabidopsis中适当的日间生长调节.
科学领域:
- 植物分子生物学 植物分子生物学
- 循环节律是指循环节律的节奏.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 植物利用昼夜时钟,与日夜周期同步,调节白天生长和发育.
- 关键的晚间时钟组件,包括伪响应调节器5 (PRR5),电缆表达式1 (TOC1) 和早期开花3 (ELF3),对于这一规定至关重要.
研究的目的:
- 调查夜间时钟组件之间的物理相互作用及其在调节阴囊延长中的作用.
- 阐明在夜间抑制植物染色体交互因子4 (PIF4) 的表观遗传机制.
主要方法:
- 在阿拉比多普西斯 (prr5-1 toc1-21 elf3-1) 中对突变表型的分析.
- 测量 hypocotyl 延伸速率的方法.
- 对PIF4基因表达的量化.
- 染色体免疫沉测试以评估PIF4位点的基因组修饰和蛋白质结合.
主要成果:
- PRR5,TOC1和ELF3之间的物理相互作用在PIF4位点建立了白天抑制色素结构.
- 这些时钟组件的突变导致PIF4表达的增加和 hypocotyl 延长,特别是在夜间.
- SWI2/SNF2-RELATED (SWR1) 染色体重塑复合物通过在PIF4位点内加入H2A.Z来调节这种抑制.
结论:
- 在晚上,PRR-EC-SWR1复合体在表观遗传上抑制PIF4的表达,从而抑制夜间阴囊延长.
- 这种机制凸显了基于染色体的调节在将昼夜时钟与植物生长反应联系起来的重要性.
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