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抗酸微的开发和表征,以缓冲酸性椎间盘微环境

Jennifer Gansau^1,2,3, Emily E McDonnell^1,2, Conor T Buckley^1,2,4,5

¹Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, The University of Dublin, Dublin, Ireland.

Journal of biomedical materials research. Part A

|May 31, 2024

概括

抗酸微囊,特别是碳酸 (CaCO3),有效中和退化的椎间盘 (IVDs) 中的酸性条件. 这种方法显示出增强基于细胞的疗法和改善IVD再生的微环境的希望.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
生物化学生物化学

背景情况:

椎间盘 (IVD) 退化以酸性微环境为特征,影响细胞功能和矩阵周转.
目前用于IVD再生的基于细胞的疗法因这些敌对的微环境条件而面临挑战.

研究的目的:

开发和描述抗酸微囊,以中和退化的IVD中的酸性状况.
评估将抗酸微囊与细胞微囊结合在用于IVD再生的混合凝系统中的治疗潜力.

主要方法:

选了五种pH中和剂,其中Mg (OH) 2和CaCO3被选为进一步研究.
抗酸酸盐微囊的制造和表征,使用电动力喷雾.
在退行性条件下,在牛椎间盘扩展模型中评估微囊的性能.

主要成果:

与其他药物相比,碳酸 (CaCO3) 显示出更高的pH中和能力,释放动力学和细胞兼容性.
在磁盘爆炸模型中,CaCO3微囊成功地提高了混合细胞凝系统的酸性核心的pH值.
据估计,CaCO3微囊的中和作用会持续数周.

结论:

抗酸微囊,特别是CaCO3,可以有效地减轻与退行性磁盘疾病相关的酸性微环境.
这项技术具有很大的潜力,可以增强IVD的基于细胞的再生策略.
开发的抗酸微囊为改善IVD再生条件提供了一个有前途的治疗方法.

关键词:

在CaCO3CO3中.(Mg) 2 (OH) 2 (Mg) 2 (OH) 2 (Mg) 2 (Mg) 2 (OH) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 3 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 2 (Mg) 3 (Mg) 2 (Mg) 2 (Mg) 3 (Mg) 2 (Mg) 2 (Mg) 3 (Mg) 3 (Mg) 3 (Mg) 3 (Mg) 4 (Mg) 3 (Mg) 4 (Mg) 3 ()缓冲区的缓冲区是一个缓冲区.它的核是脉冲的脉冲.它们的pH值为pH.

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