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对内源逆转录病毒元素的全基因组识别及其在子基因组中的活跃转录

Zheng Li^1,2, Qing Wang^1,3, Na Lv^1,4

¹CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of Microbiology, Chinese Academy of Sciences Beijing China.

|May 31, 2024

概括

在美国子基因组中发现了内源逆转录病毒 (ERV) 的活跃转录. 许多ERV位点是特定于组织的,可以影响宿主基因表达,这表明它在哺乳动物生物学中发挥了作用.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
病毒学病毒学

背景情况:

哺乳动物内源逆转录病毒 (ERV) 是古老的病毒序列,集成到宿主基因组中.
从历史上看,ERV被认为是转录无声的,但最近的发现表明某些物种的转录活跃.
哺乳动物基因组中ERVs的功能意义和转录活性在很大程度上仍未被探索.

研究的目的:

在美国子 (Neogale vison) 中进行第一个ERV位点的全基因组识别.
使用转录组数据分析这些已识别的ERV位点的转录活性和组织特异性.
研究ERV转录与宿主基因表达之间的关系.

主要方法:

在美国子基因组中对ERV位点进行全基因组扫描 (NeoERV识别).
在三种组织类型 (杏仁体,气管,肺) 中进行转录组分析,以检测活跃的NeoERV转录.
对NeoERV转录水平和宿主基因转录之间的相关性分析,特别是在重叠区域.

主要成果:

在美国子基因组中识别了365,791个NeoERV位点.
发现161,205 (44%) 的这些位点被积极转录.
观察到,超过三分之一的活跃的NeoERV位点表现出特定组织的表达模式.
发现NeoERV和宿主基因转录之间的正相关性,特别是在重叠区域.
对Env基因的分析显示,其转录率通常高于其他NeoERVs,可能与宿主免疫有关.

结论:

美国子基因组含有大量活跃转录的内源逆转录病毒 (NeoERVs).
NeoERV转录的很大一部分是组织特异的,可以与宿主基因表达相关联.
这些发现突显了哺乳动物基因组中ERVs的动态性质,并表明了潜在的功能作用,可能与宿主免疫有关.

关键词:

内源性逆转录病毒的发生.子子子子组织特异性的组织特异性.转录组 (transcriptome) 是一个转录组.

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