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广泛传播的Bathyarchaeia编码了一种新型的甲基转移酶,利用素衍生的芳香物

Tiantian Yu^1,2, Haining Hu², Xianhong Zeng²

¹School of Oceanography Shanghai Jiao Tong University Shanghai China.

|May 31, 2024

概括

海洋Bathyarchaeia可以使用新型甲基转移酶 (MT1) 降解红素. 这些微生物在沿海沉积物中普遍存在,在全球碳循环中发挥着关键作用.

科学领域:

微生物生态学微生物生态学
生物地质化学生物地质化学
分子生物学分子生物学

背景情况:

红素降解对陆地和海洋环境的全球碳循环至关重要.
浴菌是一种丰富的海洋微生物,被怀疑是介导无氧木质素分解.
由于Bathyarchaeia降解红素的确切机制尚不清楚.

研究的目的:

为了研究海洋Bathyarchaeia无氧素降解的机制.
为了识别和描述参与素单体O-脱甲基化中的酶.
为了确定在沿海沉积物中降解素的Bathyarchaeia的流行和活性.

主要方法:

从沿海沉积物中提取巴提亚基亚的丰富培养.
在无氧条件下,使用红素作为唯一的碳来源进行生长实验.
对一种新型甲基转移酶 (MT1) 的基因表达分析和异质表达.
来自各种沿海沉积物的环境DNA的元基因组分析.

主要成果:

一种丰富培养, *Candidatus* Baizosediminiarchaeum ligniniphilus (Ca. B. ligniniphilus),使用红素作为其唯一的碳来源.
这是因为. B. ligniniphilus高度表达一种新型甲基转移酶 (MT1,mtgB),它负责O-脱甲基化素单体.
MT1酶是Bathyarchaeia独特的,并证实具有O-脱甲基化活性.
*mtgB*基因在沿海沉积物中广泛存在,在东中国海沉积物中高度表达.

结论:

浴菌具有并利用新型甲基转移酶 (MT1) 进行素单体的O-脱甲基化.
这些发现揭示了由Bathyarchaeia调解的无氧素降解的关键机制.
浴菌在沿海海洋生态系统中是无处不在的和活跃的素降解剂,对碳循环做出了重大贡献.

关键词:

浴室古代古代古代古代沿海沉积物的海岸沉积物.红素降解的降解素衍生芳化合物素衍生芳化合物特定的甲基转移酶.

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