一种深海硫酸盐减少细菌通过代谢硫酸盐产生零价值硫
Rui Liu1,2,3, Yeqi Shan1,2,3,4, Shichuan Xi3,4,5
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences Qingdao China.
mLife
|May 31, 2024
概括
减少硫酸盐的细菌 (SRB) 可以通过减少硫酸盐和氧化硫化物产生零价值硫 (ZVS),由PhsA和SQR酶介导. 这一发现扩大了我们对深海环境中硫循环的理解.
科学领域:
- 地质微生物学的生物.
- 生物地质化学循环的过程
- 海洋微生物学 海洋微生物学
背景情况:
- 零价值硫 (ZVS) 是硫循环中的关键中间体,通常在深海冷中发现.
- 硫氧化细菌传统上被认为是ZVS的主要生产者.
- 硫酸盐减少细菌 (SRB) 在ZVS形成中的作用在很大程度上没有报告.
研究的目的:
- 调查SRB生产ZVS的潜力.
- 为了确定特定的微生物通路和酶参与ZVS由SRB的形成.
- 评估深海微生物群落中与ZVS产生相关的基因的流行程度.
主要方法:
- 从深海冷沉积物中分离和培养SRB.
- 生物化学分析检测ZVS的形成.
- 蛋白质组分析以确定关键酶 (PhsA和SQR).
- 在模拟深海条件下的基因表达分析.
- 深海沉积物微生物群落的元基因组学分析.
主要成果:
- 一种新型的SRB, *Oceanidesulfovibrio marinus* CS1,被分离出来,并被证明可以从硫酸盐中产生ZVS.
- 硫酸硫酸还原酶 (PhsA) 和硫化:酸氧还原酶 (SQR) 被确定为ZVS形成途径中的关键酶.
- 在深海条件下,CS1中*phsA*和*sqr*基因的表达被上调.
- *phsA*和*sqr*的同类在深海冷透微生物群落中广泛存在.
结论:
- SRB,特别是*O. marinus* CS1,可以积极产生ZVS.
- PhsA和SQR酶系统对于SRB介导的ZVS生产至关重要.
- 拥有*phsA*和*sqr*基因的SRB可能会在深海冷透生态系统中对ZVS形成作出重大贡献.
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