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bZIP转录因子ATF1调节了Trichoderma guizhouense中的蓝光和氧化应激反应

Yifan Li¹, Yanshen Li¹, Huanhong Lu¹

¹Nanjing Agricultural University, Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province Nanjing China.

|May 31, 2024

概括

转录因子ATF1对于蓝光反应和Trichoderma guizhouense的生长至关重要,它在HOG1通路的下游起作用. ATF1调节涉及光响应,发芽和抗压力的基因.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
植物病理学植物病理学

背景情况:

线素激活蛋白激酶 (MAPK) HOG通路参与真菌的光和压力信号传递.
蓝光激活了Trichoderma物种中的HOG通路.

研究的目的:

为了研究转录因子ATF1在蓝光反应中的作用和Trichoderma guizhouense中MAPK HOG1的下游信号.
阐明ATF1在真菌生长,发育和抗压力中的作用.

主要方法:

基因操纵以创建ATF1删除突变.
分析真菌生长,状发芽和光状发芽.
酵母双混合或共同免疫沉试验用于研究蛋白质相互作用.
全基因组转录组分析 (RNA-Seq) 来识别受调节的基因.

主要成果:

ATF1对于蓝光诱导的结合,植物生长和结合芽是必不可少的.
在蓝光或H2O2刺激下,HOG1与核中的ATF1相互作用.
很大一部分蓝光调节基因依赖ATF1和/或HOG1.
ATF1和HOG1在暗中诱导的状芽中具有相反的作用.
由于催化酶基因的下调,ATF1缺乏会降低氧化应激抵抗力.

结论:

ATF1作用于HOG1的下游,是Trichoderma guizhouense.中蓝光反应的关键调节者.
ATF1在真菌发育中起着关键作用,包括结合和发芽.
在这种真菌中,ATF1参与调解氧化应激抵抗.

关键词:

马普克·霍格 (MAPK HOG) 是一个三皮病的患者看起来很像.bZIP转录因子ATF1的转录因子光的反应对光的反应.氧化应激是一种氧化应激.

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