化学辅助的微分脉冲电压测量用于同时且无干扰地量化酸和咖啡酸
Antonella E Montemerlo1,2, Silvana M Azcarate2,3, José M Camiña2,3
1Instituto de Química de San Luis, "Dr Roberto A. Olsina" (INQUISAL-CONICET-UNSL), San Luis, 5700, Argentina.
概括
化学测量工具使红葡萄酒中的酸和咖啡酸可以同时通过差分脉冲电压测量和多变量曲线分辨率来确定. 这种方法提供了一种简单,快速和经济的替代方法,用于在不修改电极的情况下进行多分析.
科学领域:
- 分析化学 分析化学
- 电化学 电化学 电化学
- 化学测量 化学测量 化学测量
背景情况:
- 酸和咖啡酸是阿根廷红葡萄酒中的关键多,影响体感特性,并作为质量标志物.
- 同时量化这些多是具有挑战性的,因为它们的电化学信号的重叠是显著的.
- 现有的方法通常需要对电极进行修改或采用替代分析技术.
研究的目的:
- 开发和验证一种方法,用于在没有电极修改的情况下同时确定酸和咖啡酸.
- 应用化学测量工具,特别是多变量曲线分辨率与交替最小平方 (MCR-ALS),用于分析复杂的混合物.
- 评估该方法是否适用于葡萄酒行业的常规分析.
主要方法:
- 微分脉冲电压测量 (DPV) 实验在各种潜在步骤中进行,以生成二级数据.
- 应用了多变量曲线分辨率与交替最小正方形 (MCR-ALS) 来解决重叠信号.
- 在MCR-ALS优化过程中使用了约束,以防止旋转模两可,并确保独特的解决方案.
主要成果:
- 该研究成功地实现了酸和咖啡酸的同时定量.
- 检测极限 (LOD) 确定为酸的1.6 mg L-1和咖啡酸的7.6 mg L-1.
- 在LOD和300毫克L-1之间观察到线性依赖,表明了良好的定量性能.
结论:
- 开发的MCR-ALS方法为同时测定多提供了强大而准确的方法.
- 该方法简单,经济有效,快速,不需要电极修改,使其具有优势.
- 这种技术为葡萄酒行业的多分析提供了有希望的替代方案,特别是用于质量控制.
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