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转录因子OsWRKY11诱导低度的米,但抑制高度的米

Lirong Zhao^1,2, Yunwei Liu³, Yi Zhu^3,4

¹CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, The Chinese Academy of Sciences, Mengla, 666303, China.

Journal of integrative plant biology

|May 31, 2024

概括

WRKY转录因子OsWRKY11通过在正常条件下促进开花,并在干旱压力下延迟开花来调节大米标题日期. 这项研究揭示了OsWRKY11的存在.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学
遗传学是一个遗传学.

背景情况:

米的标题日期是区域适应和产量的关键特征.
已知 WRKY 转录因子会影响植物发育,但它们在米标题日期调节中的作用尚未完全理解.

研究的目的:

阐明了WRKY转录因子调节大米标题日期的精确机制.
为了确定参与控制大米开花时间的特定WRKY基因.

主要方法:

利用一个聚类定期间隔的平行体重复 (CRISPR) - - CRISPR相关核酶9 (Cas9) 突变库,针对大米中的 WRKY 基因.
在不同的光线条件下分析了OsWRKY11突变和过度表达线的标题日期.
研究了分子机制,包括基因表达分析和蛋白质复合体形成.

主要成果:

OsWRKY11被确定为大米标题日期的关键调节器.
OsWRKY11表现出双重调节作用,通过诱导OsMADS14/OsMADS15促进开花,并通过形成抑制Ehd1.1的复合体来延迟开花.
干旱压力会对OsWRKY11的表达产生不同的影响,导致航线的提升或延迟.

结论:

通过直接和间接的DNA结合,OsWRKY11在米头日期调节中发挥着关键作用.
OsWRKY11集成了干旱等环境信号来调节开花时间.
这些发现揭示了在涉及OsWRKY11.11的中进行标题日期控制的新机制.

关键词:

这就是OsWRKY11的原因.标题日期标题日期标题日期米米饭米饭米饭米饭.转录因子是一种转录因子.

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