如何获得和比较元基因组组装基因组
Fabio Beltrame Sanchez1, Suzana Eiko Sato Guima1, João Carlos Setubal2
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
Methods in molecular biology (Clifton, N.J.)
|May 31, 2024
概括
大基因组组装基因组 (MAGs) 提供了对未培养微生物的洞察力. 本指南详细介绍了MAG的检索,处理和分析,使用当前的工具和真实数据.
科学领域:
- 微生物生态学 微生物生态学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 基因组组装基因组 (MAG) 是从环境DNA测序中衍生出来的.
- 商业授权组为难以培养或不可能培养的微生物物种提供基因组数据.
- 由于测序技术的进步和对微生物生态学的兴趣增加,可用的MAG的数量呈指数增长.
研究的目的:
- 提供对元基因组组装基因组 (MAG) 检索和处理的基本理解.
- 为MAG分析和比较提供一个实用的,逐步指南.
- 为研究人员提供知识,以利用MAG数据的最先进工具.
主要方法:
- 从元基因组数据集中进行MAG检索.
- 组装基因组的处理和质量控制.
- 利用当代生物信息学工具进行MAG分析和比较基因组学.
主要成果:
- 使用现实世界数据集展示MAG检索和处理.
- 现有工具的实际应用,以进行全面的MAG分析.
- 促进对未培养微生物种群进行比较基因组研究.
结论:
- 在研究未培养的微生物多样性方面,MAG是至关重要的.
- 本章是进入MAG分析领域的研究人员的实用资源.
- 提出的方法和工具可以通过基因组重建对微生物群落进行强有力的研究.
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