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微观分离的连接体分布在联合组装的-友纳米纤维中

Turash Haque Pial^1,2, Yang Li^2,3, Monica Olvera de la Cruz^1,2,3,4,5

¹Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, IL 60208, USA. m-olvera@northwestern.edu.

|May 31, 2024

概括

用于抗体净化功能化的两性蛋白倾向于聚集在纤维表面. 静电排斥防止完全分离,指导先进纳米纤维的设计.

科学领域:

生物材料科学生物材料科学
超分子化学超分子化学
纳米技术纳米技术

背景情况:

两性 (PAs) 自组装成纳米纤维用于生物医学应用.
功能化PA与填充性PA的联合组装在蛋白质净化等应用中很常见.
控制联合组装纤维的同质性或分离性对于它们的功能至关重要.

研究的目的:

为了研究负电荷的填充PAs (C12VVEE) 和正电荷的功能化PAs (C12VVKK-OEG4-Z33) 的联合组装行为.
了解控制在纳米尺度上的同质混合与分离的因素,用于抗体净化应用.
将实验观测与有关自组装模式的理论预测联系起来.

主要方法:

联合组装的C12VVEE和C12VVKK-OEG4-Z33类两.
对产生的纳米纤维进行表面分析,以观察分子分布.
将实验结果与自组装的理论模型进行比较.

主要成果:

功能化的C12VVKK-OEG4-Z33配体在纳米纤维表面呈现集群和局部分离.
由于近距离的吸引力,中性Z33元件在散装溶液中形成聚合物.
纤维表面C12VVKK-OEG4-Z33的完全分离受到静电排斥的阻碍,防止类似电荷的大范围.

结论:

短距离的吸引力和远距离的静电排斥之间的相互作用决定了圆柱形PA表面的图案形成.
相反电荷的PAs的联合组装导致复杂的表面图案,而不是均的混合物.
获得的见解对于设计功能化纳米纤维,用于医学和化学的有针对性的应用非常有价值.

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