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为Campylobacter hepaticus开发一种选择性培养方法

Sheaaz G J Sakur1,2, Sarah L Williamson2, Anthony Pavic2

  • 1School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camden, New South Wales, Australia.

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开发选择性媒介对Campylobacter hepaticus (C. hepaticus) 对于诊断的斑点性肝病 (SLD) 是非常重要的. 带有血液的博尔顿,补充了特定的抗微生物药物,显示出对隔离这种病原体最有希望的方法.

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科学领域:

  • 兽医微生物学 兽医微生物学
  • 禽类疾病诊断 诊断 禽类疾病诊断
  • 细菌学 细菌学是一门学科.

背景情况:

  • 坎皮洛巴克特肝炎在中引起斑点性肝病 (SLD),导致死亡率和生产损失.
  • 目前对C. hepaticus的隔离方法依赖于胆汁样本,阻碍了便和环境诊断.
  • 需要选择性生长基来改善SLD的诊断和影响评估.

研究的目的:

  • 评估不同的介质配方,用于选择性隔离和生长Campylobacter hepaticus.
  • 为了确定C. hepaticus分离物的抗微生物敏感性概况.
  • 为了确定一个最佳的选择性介质,用于例行诊断.

主要方法:

  • 用血液和/或胆汁在各种基 (HBA,SBA,博尔顿,普雷斯顿,BHI) 上对五种C. hepaticus分离物的生长试验.
  • 在42°C的微气友条件下化10天.
  • 使用HBA板对14种药物进行抗菌敏感性测试.

主要成果:

  • 补充血液的博尔顿粉支持了C. hepaticus的最佳生长.
  • 大脑心脏输液 (BHI) 介质和添加胆汁抑制了C. hepaticus的生长.
  • 所有分离物都对三甲和万科米辛有耐药性;四个也对利芬素和巴西特拉辛有耐药性.

结论:

  • 带着血液的博尔顿是C. hepaticus生长的最有效的基质介质.
  • 选择性介质包含万科米辛和三甲可能适合C.肝炎菌分离.
  • 胆汁添加有害于C. hepaticus的隔离,应该避免.