相关实验视频

Updated: Jun 25, 2025

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

长期鼻炎与鼻的细菌和真菌群体

Eray Uzunoğlu¹, Ayşe Kalkancı², Esra Kılıç²

¹Department of Otorhinolaryngology, Izmir Ekol Hospital, İzmir, Turkey.

|May 31, 2024

概括

患有慢性鼻炎与鼻息肉 (CRSwNP) 的患者的鼻腔微生物组有显著的变化. 与健康个体相比,CRSwNP患者的Corynebacterium细菌较少,菌菌较多.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
耳鼻喉科耳鼻喉科耳鼻喉科

背景情况:

带鼻息肉的慢性鼻炎 (CRSwNP) 涉及复杂的炎症过程.
环境因素,特别是微生物,显著影响粘膜炎症.
了解鼻腔微生物群对于CRSwNP的发病过程至关重要.

研究的目的:

研究CRSwNP患者鼻腔中独特的微生物群落.
为了比较CRSwNP患者和健康对照人群之间的细菌和真菌概况.
为了确定与CRSwNP发展相关的微生物差异.

主要方法:

16S rRNA和ITS测序来自18名CRSwNP患者和10名健康对照的鼻子拭子样本.
DNA提取,使用Swift Amplicon 16S + ITS套件编制库,以及Illumina Miseq测序.
使用QIIME与Silva和UNITE数据库进行细菌和真菌识别的生物信息分析.

主要成果:

在所有群体中占主导地位的细菌包括Firmicutes,Proteobacteria和Actinobacteria.
在CRSwNP患者中,Corynebacterium的发病率较低,而Streptococcus,Moraxella,Rothia,Micrococcus,Gemella和Prevotella的发病率更高.
马拉塞西亚是两组中占主导地位的真菌属,马拉塞西亚和Corynebacterium丰富度之间存在正相关性.

结论:

与对照组相比,CRSwNP患者的鼻子微生物组表现出更大的个体间变异性.
减少Corynebacterium的丰富性是区分CRSwNP患者的一个关键特征.
马拉塞西亚是主要的鼻,其存在与Corynebacterium水平相关.

更多相关视频

Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways

Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways

Published on: January 13, 2016

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

相关概念视频

Chronic Pharyngitis

Chronic Pharyngitis

Chronic pharyngitis refers to persistent inflammation of the pharyngial mucosa.
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation

Cystic Fibrosis: Management

Cystic Fibrosis: Management

Cystic fibrosis (CF) is an autosomal recessive disorder that predominantly affects individuals of Northern European descent, occurring at a rate of 1 in 3500. It is caused by a genetic mutation in a gene on chromosome 7, most commonly the ΔF508 mutation, that codes for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This results in thicker mucus secretions and obstruction pathologies in multiple organs, including the lungs and sinuses.
Sinus disease and chronic...