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环_0000006和环_0000160调节大动脉剖析进展中的hsa-let-7e-5p/UBQLN4轴

Yong Liu¹, Liang Wang¹, Dongyun Lei²

¹Department of Cardiovascular Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.

|May 31, 2024

概括

循环RNAs (circRNAs) circ_0000006和circ_0000160通过影响血管光滑肌细胞来促进大动脉解剖 (AD) 的进展. 针对这些circRNAs可能为AD提供治疗策略.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
在RNA生物学,RNA生物学.

背景情况:

大动脉动脉瘤 (AA) 和大动脉解剖 (AD) 是严重的血管疾病,发病率和死亡率不断增加.
非编码RNAs,包括circRNAs和microRNAs (miRNAs),都与AA和AD的发病有关.

研究的目的:

为了确定circRNA-miRNA-mRNA轴调节从AA到AD的过渡.
调查关键分子在阿尔茨海默病发展中的功能作用.

主要方法:

来自AA和AD患者的血样本接受了circRNA,miRNA和mRNA测序.
在体外研究中使用了AD细胞模型与血管光滑肌细胞 (VSMCs).
在体内研究中使用了AD的动物模型.

主要成果:

在AD样本中观察到升高的circ_0000006和circ_0000160,以及降低的hsa-let-7e-5p.
在circ_0000006和circ_0000160中,静音减弱了PDGF诱导的VSMC变化;通过hsa-let-7e-5p抑制,这部分逆转了.
过度表达的乌比基林4 (UBQLN4) 抵消了hsa-let-7e-5p的作用,表明UBQLN4是下游的媒介.
在动物AD模型中,circ_0000006和circ_0000160的 Knockdown 已证明具有保护作用.

结论:

上调的circ_0000006和circ_0000160通过VSMC功能障碍促进AA向AD的进展.
在AD病变发生过程中,hsa-let-7e-5p/UBQLN4轴至关重要.
准circ_0000006和circ_0000160为AD预防提供了潜在的治疗途径.

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