埃莫丁纳米囊通过调节巨细胞极化中的脂质代谢重编程来抑制急性胰腺炎
Huiyi Song1, Jianbin Zhang2, Ni Lou1
1Clinical Laboratory of Integrative Medicine, First Hospital affiliated to Dalian Medical University, Dalian, Liaoning, PR China.
曼诺糖结合基托桑涂层脂质纳米囊 (M-CS-E-LNC) 改善了严重急性胰腺炎 (SAP) 治疗的埃莫丁输送. 这种有针对性的方法提高了生物可用性,并通过CPT1激活了巨细胞两极分化,为SAP提供了一个新的治疗策略.
科学领域:
- 纳米技术纳米技术
- 药理学 药理学是指药理学的学科.
- 免疫学 免疫学 免疫学
背景情况:
- 埃莫丁是一种来自传统中国草药的化合物,具有抗炎性质,有利于严重急性胰腺炎 (SAP).
- 埃莫丁的临床应用受到溶解性差,高毒性和胰腺保留时间短的限制.
- 之前的研究表明,emodin在缓解SAP炎症方面具有潜力.
研究的目的:
- 为SAP治疗开发具有增强生物可用性和向性巨细胞递送的emodin纳米囊 (M-CS-E-LNC).
- 研究M-CS-E-LNC在调节巨细胞极化和治疗SAP中的机制.
- 通过向巨细胞来探索受控的埃莫丁释放.
主要方法:
- M-CS-E-LNC是使用修改相位逆转方法准备的.
- 在SAP的细胞和小鼠模型中,使用ELISA,IHC和IF评估了抗炎疗效.
- 使用IVIS成像和HPLC研究了向释放和胰腺积累;脂管学和基因沉默 (CTP1的shRNA) 用于机械研究.
主要成果:
- 在SAP小鼠中,M-CS-E-LNC显著降低了巨细胞中的炎症媒介和血清标记物 (氨酶,TNF-α,IL-6).
- 埃莫丁在胰腺和胃肠组织中选择性积累,表明有针对性的释放.
- M-CS-E-LNC治疗提高了卡尼丁棕转移酶1 (CPT1) 表达的调节,促进了M2巨细胞的两极分化.
结论:
- 在SAP中,M-CS-E-LNC通过增强巨细胞两极分化,证明了改善生物可用性,溶解性和治疗疗效.
- 鉴定出CPT1是严重急性胰腺炎的潜在新型治疗标.
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