在PDCoV感染期间,与IFITM3蛋白相关联的新型相互作用蛋白通过协同亲和性净化和质谱学识别出了与IFITM3蛋白相关的新型相互作用蛋白
Cheng Li1, Xiao-Ran Guo1, Zhi-Min Dong1
1Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China; Tianjin Observation and Experimental Site of National Animal Health, Tianjin 300381, China; National Data Center of Animal Health, Tianjin 300381, China.
干扰素诱导的跨膜3 (IFITM3) 蛋白活性由IFN-α增强,减少猪三角冠状病毒 (PDCoV) 感染. IFITM3与Rab9a相互作用,在PDCoV感染期间扩大其已知的细胞作用.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 干扰素诱导的转膜3 (IFITM3) 是一种具有已知的抗病毒特性的蛋白质.
- 在猪三角型冠状病毒 (PDCoV) 感染中IFITM3的特定机制尚不清楚.
研究的目的:
- 研究IFITM3在PDCoV感染中的作用和机制.
- 在PDCoV感染期间识别与IFITM3相互作用的蛋白质.
主要方法:
- 用IFN-α治疗诱导IFITM3活动.
- 过度表达IFITM3以评估其对PDCoV复制的影响.
- 双联亲和性净化,然后进行质谱学 (TAP/MS) 来识别IFITM3相互作用蛋白.
- 基因本体学 (GO) 标注,KEGG通路分析和蛋白质相互作用网络分析.
- 同焦点显微镜用于IFITM3和Rab9a的免疫光同位分析.
主要成果:
- IFN-α治疗上调了IFITM3,减轻了PDCoV感染.
- IFITM3过度表达以剂量依赖的方式抑制了PDCoV复制.
- 总共有362个细胞蛋白被确定与IFITM3感染后相关.
- 发现IFITM3与Rab9a具有特殊的相互作用,Rab9a在PDCoV感染期间表现出增强的局部化.
结论:
- IFITM3在对抗PDCoV感染方面发挥着重要作用.
- 这项研究扩展了IFITM3.3已知的蛋白相互作用网络.
- IFITM3可能参与PDCoV感染相关的多个细胞过程,包括与Rab9a的相互作用.
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